1. Academic Validation
  2. Isoprenylated flavonoids from the root bark of Morus alba and their hepatoprotective and neuroprotective activities

Isoprenylated flavonoids from the root bark of Morus alba and their hepatoprotective and neuroprotective activities

  • Arch Pharm Res. 2015 Nov;38(11):2066-75. doi: 10.1007/s12272-015-0613-8.
Jae-Woo Jung 1 Won-Min Ko 2 Ji-Hae Park 1 Kyeong-Hwa Seo 1 Eun-Ji Oh 1 Dae-Young Lee 3 Dong-Sung Lee 4 Youn-Chul Kim 2 Dong-Wook Lim 5 Daeseok Han 5 Nam-In Baek 6
Affiliations

Affiliations

  • 1 Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.
  • 2 Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, 570-749, Republic of Korea.
  • 3 Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 369-873, Republic of Korea.
  • 4 Inha Research Institute for Medical Sciences, Inha University School of Medicine, Incheon, 400-712, Republic of Korea.
  • 5 Division of Matabolism and Functionality Research, Korea Food Research Institute, Sungnam, 463-746, Republic of Korea.
  • 6 Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea. nibaek@khu.ac.kr.
Abstract

A new isoprenylated flavonoid, 2S-5,7,2',4'-tetrahydroxy-3',5'-di-(γ,γ-dimethylallyl)flavanone, sanggenol Q (1), along with seven known isoprenylated Flavonoids, sanggenol A (2), sanggenol L (3), kuwanon T (4), cyclomorusin (5), sanggenon F (6), sanggenol O (7), and sanggenon N (8), three known Diels-Alder type adducts, sanggenon G (9), mulberrofuran G (10), and mulberrofuran C (11), and a known benzofuran, moracin E (12), were isolated from the root bark of Morus alba using silica gel, ODS, and Sephadex LH-20 column chromatography. Chemical structures were determined based on spectroscopic data analyses including NMR, MS, CD, and IR. For the first time, compounds 1 and 7 were isolated from the root bark of M. alba. All compounds were evaluated for hepatoprotective activity on t-BHP-induced oxidative stress in HepG2 cells and neuroprotective activity on glutamate-induced cell death in HT22 cells. Compounds 1, 4, 8, 10, and 11 showed protective effects on t-BHP-induced oxidative stress with EC50 values of 6.94 ± 0.38, 30.32 ± 6.82, 23.45 ± 4.72, 15.31 ± 2.21, and 0.41 ± 0.48 μM, respectively, and compounds 1, 2, 10, 11, and 12 showed protective effects on glutamate-induced cell death with EC50 values of 5.54 ± 0.86, 34.03 ± 7.71, 19.71 ± 0.71, 16.50 ± 7.82, and 1.02 ± 0.13 μM, respectively.

Keywords

HT22; HepG2; Hepatoprotective activity; Isoprenylated flavonoid; Morus alba L.; Neuroprotective activity.

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