1. Academic Validation
  2. Comparison of binding interaction between β-lactoglobulin and three common polyphenols using multi-spectroscopy and modeling methods

Comparison of binding interaction between β-lactoglobulin and three common polyphenols using multi-spectroscopy and modeling methods

  • Food Chem. 2017 Aug 1;228:143-151. doi: 10.1016/j.foodchem.2017.01.131.
Jingjing Jia 1 Xin Gao 1 Minghao Hao 1 Lin Tang 2
Affiliations

Affiliations

  • 1 School of Life Science, Shandong Normal University, Jinan, PR China.
  • 2 School of Life Science, Shandong Normal University, Jinan, PR China. Electronic address: tanglin@sdnu.edu.cn.
Abstract

Tea, coffee and fruit in dairy products are rich in Polyphenols. The interaction mechanism between β-lactoglobulin (β-LG) and chlorogenic acid (CGA), ferulic acid (FA) and epigallocatechin-3-gallate (EGCG) was investigated. Fluorescence experiments proved that Polyphenols quenched β-LG fluorescence strongly in static mode and EGCG had stronger binding affinity toward β-LG than CGA and FA. The main interaction force of EGCG binding with β-LG was different from CGA and FA. Furthermore, circular dichroism and fourier transform infrared data indicated that Polyphenols changed β-LG secondary structure inducing a-helix to β-structures transition. The surface hydrophobicity of β-LG was also changed slightly by them according to surface hydrophobicity and particle size experiments. These results showed that the interaction mechanism of β-LG with phenolic acid esters was different from it with phenolic acids. Besides, Polyphenols had impact on the structure and functionality of β-LG, which would be valuable in dairy processing industry.

Keywords

8-Anilino-1-naphthalenesulfonic acid (PubChem CID: 1369); Chlorogenic acid; Chlorogenic acid (PubChem CID: 1794427); Circular dichroism; Disodium hydrogen phosphate (PubChem CID: 24203); Epigallocatechin-3-gallate; Epigallocatechin-3-gallate (PubChem CID: 65064); Ferulic acid; Ferulic acid (PubChem CID: 445858); Fluorescence; Monosodium phosphate (PubChem CID: 23672064); β-Lactoglobulin.

Figures
Products