Inhibitory effect of α-terpinyl acetate on cytochrome P450 2B6 enzymatic activity

Human Cytochrome P450 2B6 is an important hepatic Enzyme for the metabolism of xenobiotics and clinical drugs. Recently, more attention has been paid to P450 2B6 because of the increasing number of drugs it metabolizes. It has been known to interact with terpenes, the major constituents of the essential oils used for various medicinal purposes. In this study, the effect of monoterpenes on P450 2B6 catalytic activity was investigated. Recombinant P450 2B6 was expressed in Escherichia coli and purified using Ni-affinity chromatography. The purified P450 2B6 Enzyme displayed bupropion hydroxylation activity in gas-mass spectrometry (GC-MS) analysis with a k_cat of 0.5 min^-1 and a K_m of 47 μM. Many terpenes displayed the type I binding spectra to purified P450 2B6 Enzyme and α-terpinyl acetate showed strong binding affinity with a K_d value of 5.4 μM. In GC-MS analysis, P450 2B6 converted α-terpinyl acetate to a putative oxidative product. The bupropion hydroxylation activity of P450 2B6 was inhibited by α-terpinyl acetate and its IC₅₀ value was 10.4 μM α-Terpinyl acetate was determined to be a competitive inhibitor of P450 2B6 with a K_i value of 7.6 μM. The molecular docking model of the binding site of the P450 2B6 complex with α-terpinyl acetate was constructed. It showed the tight binding of α-terpinyl acetate in the active site of P450 2B6, which suggests that it could be a competitive substrate for P450 2B6.