1. Academic Validation
  2. miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR

miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR

  • Onco Targets Ther. 2018 Aug 30;11:5293-5302. doi: 10.2147/OTT.S173416.
Lansheng Zhang 1 2 Yanwei Chen 3 Hui Wang 3 Xia Zheng 2 Caihong Li 2 Zhengxiang Han 3
Affiliations

Affiliations

  • 1 Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
  • 2 Department of Radiation Oncology, the Second Affiliated Hospital of Xuzhou Medical University, Xuzhou, People's Republic of China.
  • 3 Department of Oncology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, People's Republic of China, zhxh_xmu@163.com.
Abstract

Background: The roles and related mechanism of miR-376a in breast Cancer cell progression are unclear.

Methods: Kaplan-Meier plotter analysis was used to analyze the correlation between miR-376a and the overall survival (OS) of breast Cancer patients. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect miR-376a level in breast Cancer cells. Cell viability, transwell migration and invasion, and cell Apoptosis were constructed to investigate the effects of miR-376a on breast Cancer cells. Luciferase reporter and RNA immunoprecipitation (RIP) were used to explore the targeting of miR-376a on NRP-1.

Results: miR-376a expression was positively correlated with the overall survival of breast Cancer patients, and significantly decreased in breast Cancer cells. Functionally, miR-376a over-expression suppressed cell proliferation, migration and invasion, and promoted cells Apoptosis. Additionally, miR-376a could directly target NRP-1 and exerted its effect through NRP-1.

Conclusion: miR-376a could suppress breast Cancer cell progression via directly targeting NRP-1.

Keywords

NRP-1t; Wn/β-catenin migration; breast cancert; miR-376at.

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