Cell-Based Biosensor with Dual Signal Outputs for Simultaneous Quantification of Phenylacetic Acid and Phenylethylamine

Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous Auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of Bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

E. coli; FeaR regulon; aromatic acids; auxin; phenylacetic acid; phenylethylamine.