2. Academic Validation
  3. Detection of viral components in exosomes derived from NDV-infected DF-1 cells and their promoting ability in virus replication

Detection of viral components in exosomes derived from NDV-infected DF-1 cells and their promoting ability in virus replication

  • Microb Pathog. 2019 Mar;128:414-422. doi: 10.1016/j.micpath.2018.12.047.
Xiaohong Xu 1,  Jing Qian 2,  Jiaxin Ding 1,  Jindou Li 1,  Fulong Nan 1,  Weiqi Wang 1,  Qi Qin 1,  Yidong Fei 1,  Cong Xue 3,  Jianzhong Wang 4,  Renfu Yin 1,  Zhuang Ding 5
Affiliations

Affiliations

  • 1 Laboratory of Infectious Diseases, College of Veterinary Medicine, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun, 130062, China.
  • 2 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences / Key Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture, Nanjing, 210014, China.
  • 3 College of Agriculture and Forestry Science, Linyi University, Shuangling Road, Linyi City, 276005, Shandong Province, China.
  • 4 Engineering Research Center of Jilin Province for Animals Probiotics, College of Animal Science and Technology, Jilin Agriculture University, Changchun, 130118, China.
  • 5 Laboratory of Infectious Diseases, College of Veterinary Medicine, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun, 130062, China. Electronic address: dingzhuang@jlu.edu.cn.
PMID: 30597256 DOI: 10.1016/j.micpath.2018.12.047
Abstract

Exosomes are micro messengers encapsulating RNA, DNA, and proteins for intercellular communication associated with various physiological and pathological reactions. Several viral Infection processes have been reported to pertain to exosomal pathways. However, because of the difficulty in obtaining avian-sourced exosomes, avian virus-related exosomes are scarcely investigated. In this study, we developed a protein A/G-correlated method and successfully obtained the Newcastle disease virus-related exosome (NDV Ex). These exosomes promoted NDV propagation, proven by both GW4869-mediated deprivation and exosomal supplementation. Viral structural proteins NP and F were detected in the NDV Ex and further investigation indicated that the NP protein can be transferred to DF-1 cells through exosomes. The intracellular NP protein exhibited viral replication-promoting and cytokine-suppressing abilities. Therefore, NDV Infection produces exosomes, which transfer viral NP protein and promote NDV Infection, emphasizing the importance of exosomes in an NDV Infection.

Keywords

Exosome; Infection; Newcastle disease virus (NDV); Nucleoprotein (NP); Protein A/G.

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