2. MCE Kits
  3. Protein Biology
  4. Protein Purification
  5. Magnetic Beads
  6. Protein A/G Magnetic Beads

Protein A/G Magnetic Beads 

蛋白 A/G 磁珠

Cat. No.: HY-K0202
Manual COA SDS 技术支持

蛋白 A/G 磁珠为 IP、Co-IP 和 ChIP 实验提供了一种快速便捷的方法。

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample (200 μL)   Apply now  
1 mL ￥455 In-stock
5 mL ￥1950 In-stock

* Please select Quantity before adding items.

MCE 顾客使用本产品发表的 915 篇科研文献

IP

    purchased from MCE. Usage Cited in: Cell Mol Immunol. 2023 Mar;20(3):292-304.  [Abstract]

    Immunoblot analysis of protein immunoprecipitation (IP) with antibodies against FXYD3 or TRAF3 in HaCaT cells treated with IL-17A (100 ng/ml).

    purchased from MCE. Usage Cited in: Cell Res. 2025 Mar;35(3):165-185.  [Abstract]

    Immunoprecipitation (IP) analysis of binding between Raptin and GRM3 VFT or transmembrane domain (TMD) of GRM3 in hypothalamic GT1-7 neurons. The cell lysate of hypothalamic GT1-7 neurons with human GRM3-VFT or human GRM3-TMD overexpression was incubated with PBS or His-Raptin.

    purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2024 Dec;44(12):1391-1413.  [Abstract]

    Protein A/G Magnetic Beads (4 h). The interaction between LMP1‐GFP and ALIX‐MYC was detected by a co‐IP assay.

    purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2024 Sep 26;9(1):253.  [Abstract]

    Protein A/G Magnetic Beads (30 µL) were conjugated with antibodies and lysis buffer at 4 °C. Later, the protein samples were incubated with preconjugated beads overnight at 4 °C on a rotating wheel.
    Coimmunoprecipitation of GRP75, ANT2, and UCP1. HEK293T cells were co-transfected with Myc-GRP75, ANT2, and Flag-UCP1. Protein extracts were immunoprecipitated with antibodies against Myc-tag or Flag-tag, followed by immunoblotting with the indicated antibodies.

    purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2023 May;43(5):582-612.  [Abstract]

    Protein A/G Magnetic Beads were washed four times with 0.5% PBS‐Triton X‐100. The antibody solution was then added, and the magnetic beads and antibodies were incubated for 1 h at 25°C on a flip mixer. The antibody‐conjugated magnetic beads were washed four times with 0.5% PBS‐Triton X‐100. Prepared protein supernatant was then added, and the antibodies and proteins conjugated to the magnetic beads were incubated for 2 h at 4°C on a flip mixer. After washing with 0.5% PBS‐Triton X‐100, 1 × SDS‐P

    purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2023 Apr;43(4):480-502.  [Abstract]

    The cell lysate extracted from ccRCC cells was incubated in Protein A/G Magnetic Beads with primary antibodies or IgG overnight at 4°C with shaking. The immune complexes were incubated with protein A/G magnetic beads for 2 h at room temperature followed by washing with PBST (PBS + 0.05% Tween 20) to remove the unbound immune complexes.
    (H) The DBT/YAP‐ANXA2 interaction was determined by co‐IP assays in A498 and CAKI‐1 cells with Myc‐ANXA2 overexpression.

    purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2023 Apr;43(4):435-454.  [Abstract]

    Cells lysates were prepared by using NP40 lysis buffer containing PMSF and phosphatase inhibitor cocktail. Anti‐SHP1 (1:100) or anti‐Flag (1:100) were pre‐mixed with Protein A/G Magnetic Beads for 2 h at 4°C.
    Co‐IP analysis of SHP1‐interacted STING and TRAF6 under different treatment regimens

    purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2021 Dec;41(12):1354-1372.  [Abstract]

    Immunoprecipitation of MAGE‐C3 and IFNGR1 from digitonin lysates in KYSE30 cells with or without IFN‐γ.

    purchased from MCE. Usage Cited in: Nat Metab. 2024 Aug;6(8):1505-1528.  [Abstract]

    Protein A/G Magnetic Beads (50 μL; 2 h). Immunoblotting shows Kbhb or Kac of ALDOB in livers from male mice fed a control diet or KD for 5 weeks. Immunoprecipitation was performed with a pan-Kbhb or a pan-Kac antibody from mouse liver whole-cell lysates.

    purchased from MCE. Usage Cited in: Neuro Oncol. 2024 Jan 5;26(1):137-152.  [Abstract]

    The supernatants were immunoprecipitated with antibodies prebound with Protein A/G Magnetic Beads.
    Co-IP between ADAM22 and ITGB1 in GH3 and AtT20 cells.

      Protein A/G Magnetic Beads purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2024 Sep 26;9(1):253.  [Abstract]

      Customer Validation
      Protein A/G Magnetic Beads (30 µL).Coimmunoprecipitation of GRP75, ANT2, and UCP1. HEK293T cells were co-transfected with Myc-GRP75, ANT2, and Flag-UCP1. Protein extracts were immunoprecipitated with antibodies against Myc-tag or Flag-tag, followed by immunoblotting with the indicated antibodies.

      Protein A/G Magnetic Beads purchased from MCE. Usage Cited in: Cell Res. 2025 Jan 29.  [Abstract]

      Customer Validation
      Immunoprecipitation (IP) analysis of binding between Raptin and GRM3 VFT or transmembrane domain (TMD) of GRM3 in hypothalamic GT1-7 neurons. The cell lysate of hypothalamic GT1-7 neurons with human GRM3-VFT or human GRM3-TMD overexpression was incubated with PBS or His-Raptin.

      Protein A/G Magnetic Beads purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2024 Oct 14.  [Abstract]

      Customer Validation
      Protein A/G Magnetic Beads (4 h). The interaction between LMP1‐GFP and ALIX‐MYC was detected by a co‐IP assay.

      Protein A/G Magnetic Beads purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2023 Apr 2.  [Abstract]

      Customer Validation
      MDA‐MB‐231 and SKBRS parent cell lysates were immunoprecipitated with anti‐YAP1, anti‐VPS4B, or anti‐CHMP2B antibodies, and YAP1 binding to VPS4B and CHMP2B was examined with WB analysis.

      Protein A/G Magnetic Beads purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2023 Mar 1.  [Abstract]

      Customer Validation
      The cell lysate extracted from ccRCC cells was incubated in Protein A/G Magnetic Beads with primary antibodies or IgG overnight at 4°C with shaking. The immune complexes were incubated with protein A/G magnetic beads for 2 h at room temperature followed by washing with PBST (PBS + 0.05% Tween 20) to remove the unbound immune complexes.
      (H) The DBT/YAP‐ANXA2 interaction was determined by co‐IP assays in A498 and CAKI‐1 cells with Myc‐ANXA2 overexpression.

      • 描述及优势

      • 储存条件 & 期限

      • 操作流程

      • 包装

      • 文件资料

      描述及优势

      蛋白 A/G 磁珠提供了一种利用亲和结合进行蛋白质磁分离的快速而方便的方法。MCE 蛋白质 A/G 磁珠通常用于从血清、细胞培养上清或腹水中分离抗体，以及从细胞或组织提取物中免疫沉淀和共免疫沉淀抗原。

      产品优势

      1. 免疫沉淀时只需少量磁珠。

      2. 方便省时。

      3. 非特异性结合率低。

      4. 样品损失少。

      5. 蛋白质结合能力高达 0.7 mg/mL。

      6. 稳定。
      储存条件 & 期限

      4°C，2 年

      请勿离心、干燥或冷冻磁珠。
      操作流程

      1. 抗原样品制备

      根据不同的样品选择合适的处理方法

      2. 磁珠预处理

      2.1 充分重悬磁珠.

      2.2 取 25-50 μL 蛋白质 A/G 磁珠转移到 1.5 mL EP 管中（用量量可根据需要调整）。

      2.3 加入 400 μL 结合/洗涤缓冲液，充分重悬磁珠。将 EP 管放入磁力架上，磁性分离。弃上清液。重复此步骤 2 次。

      3. 抗体与磁珠结合

      3.1 使用结合/洗涤缓冲液将抗体稀释至终浓度为 5-50 μg/mL 。

      3.2 将稀释好的 400 μL 抗体加入步骤 2 处理好的磁珠中，充分混悬，置于翻转混合仪孵育 (室温，30 mins；4°C，2 hours)。

      3.3 磁性分离，收集磁珠，上清液收集于新的 EP 管中，以备后续使用。

      3.4 加入 400 μL 结合/洗涤缓冲液，充分混悬磁珠，磁性分离，弃上清；重复洗涤 4 次。

      4. 抗原与抗体-磁珠复合物结合

      4.1：加入 400 μL 抗原样品准备的抗原样品，充分混悬，置于翻转混合仪孵育 (室温，30 mins；4°C，2 hours) ，磁性分离，弃上清。

      4.2 加入 400 μL 结合/洗涤缓冲液 充分重悬磁珠，磁性分离，弃上清；重复洗涤 4 次。

      5. 抗原洗脱

      本说明书提供以下两种抗原洗脱方案，操作者可根据后期检测的需要选择不同的抗原洗脱方法。

      a. 变性洗脱法：此方法洗脱的样品适用于 SDS-PAGE 检测。 步骤：分离磁珠，弃上清，向磁珠中加入 25-50 μL 1×SDS-PAGE Loading Buffer 混合均匀，95°C 加热 5 mins。分离磁珠，收集上清，进行 SDS-PAGE检测。

      b. 非变性洗脱法：此方法洗脱的样品保持原有的生物活性，可用于后期功能 分析。 步骤：分离磁珠，弃上清，向磁珠中加入 25-50 μL 洗脱缓冲液，室温孵育 10 mins；分离磁珠，收集上清至新的 EP 管，并立即滴入总体积 1/10 体积的中和缓冲液 (0.1 M NaOH)，将洗脱产物 pH 调节至中性，样品用于后期功 能分析。

      注: 本步骤洗脱的抗原为抗原-抗体复合物，如操作者需要单独洗脱目标抗原，推荐使用交联剂，并按相关实验说明操作。
      包装
      Components HY-K0202-1 mL HY-K0202-5 mL
      Protein A/G Magnetic Beads 1 mL 1 mL × 5
      文件资料

      Manual (470 KB) SDS (108 KB)
      Help & FAQs
      • Do most proteins show cross-species activity?

        Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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