1. MCE Kits
  2. Protein Biology
  3. Protein Purification
  4. Magnetic Beads
  5. Protein A/G Magnetic Beads

Protein A/G Magnetic Beads 

蛋白 A/G 磁珠

Cat. No.: HY-K0202
Manual SDS

Protein A/G Magnetic Beads 为 IP、Co-IP 和 ChIP 实验提供了一种快速便捷的方法。

Protein A/G Magnetic Beads

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内


3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample (200 μL)   Apply now  
1 mL ¥455 In-stock
5 mL ¥1950 In-stock

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

& Advantages

The MCE Protein A/G Magnetic Beads are typically used for isolating antibodies from serum, cell culture supernatant or ascites and for immunoprecipitation and co-immunoprecipitation of antigens from cell or tissue extracts. Protein A/G Magnetic Beads contain a recombinant Protein A/G that combines the IgG binding domains of both Protein A and Protein G.

During immunoprecipitation, only a small amount of magnetic beads are needed, and the non-specific binding is low.

•   Convenient and time saving.

•   Low non-specific binding.

•   Minimal sample loss.

•   Antibody binding capacity up to 0.5-0.8 mg/mL.

•   Stable, one bottle solution.


Stored at 4°C, and is stable for up to 2 years.

Do not centrifuge, dry or freeze the magnetic beads.


1.   Preparation of Magnetic Beads

1.1   Resuspend the Magnetic Beads in the vial (tilt and rotate for 2 minutes or gently pipette for 10 times).

1.2   Transfer 25-50 μL of Protein A/G Magnetic Beads into a 1.5 mL tube (Transfer amount may be adjusted as required).

1.3   Add 400 μL of binding/wash buffer to the beads and gently pipette to mix. Place the tube into a magnetic stand to collect the beads against the side of the tube (Hereinafter referred to as magnetic separation). Remove and discard the supernatant. Repeat this step for 2 times.

2.   Binding of Antibody

2.1   Dilute antibody (Ab) to the final concentration of 5-50 μg/mL with binding/wash buffer. The optimal amount of Ab may be adjusted as required.

2.2   Add 400 μL of diluted Ab to the Protein A/G Magnetic Beads. Rotate tube for 30 minutes at room temperature or 2 hours at 4°C.

2.3   Perform magnetic separation. Transfer the supernatant into a new tube for further analysis, if desired. The supernatant is the non-binding fraction.

2.4   Add 400 μL of binding/wash buffer to the beads and gently pipette to mix. Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 4 times.

3.   Immunoprecipitation of Target Antigen

3.1   Remove the tubes from the magnetic separator and add your sample containing the antigen (Ag) (typically 5-50 μg in 400 μL binding/wash buffer) and gently pipette to resuspend the Protein A/G Magnetic Beads-Ab complex.

3.2   Incubate with rotation for 30 minutes at room temperature or 2 hours at 4°C to allow Ag to bind to the Protein A/G Magnetic Beads-Ab complex.

3.3   Perform magnetic separation. Remove and discard the supernatant.

3.4   Wash the Magbeads-Ab-Ag complex 5 times using 400 μL binding/wash buffer for each wash. Perform magnetic separation between each wash, remove supernatant and resuspend by gentle pipetting.

3.5   Resuspend the Protein A/G Magnetic Beads-Ab-Ag complex in 400 μL binding/wash buffer and transfer the bead suspension into a clean tube. This is recommended to avoid co-elution of the proteins bound to the tube wall.

4.   Elution

This is a non-denaturation elution method.

4.1   Perform magnetic separation and remove the supernatant. Add 400 μL of binding/wash buffer into the tube and rotate for 5 minutes. Perform magnetic separation for 1 minute and remove the supernatant. Then add 25-50 μL elution buffer into the tube with magnetic beads-Ab-Ag complex, rotate for 5 minutes.

4.2   Perform magnetic separation, collect the supernatant.

4.3   The final solution can be used as samples for denaturing SDS-PAGE. Or the elution can be adjusted to neutral pH with neutralization buffer immediately and used for further analysis.

Components HY-K0202-1 mL HY-K0202-5 mL
Protein A/G Magnetic Beads 1 mL 1 mL × 5
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.


Your information is safe with us. * Required Fields.



* 需求量:

* 客户姓名:


* Email:

* 电话:


* 公司或机构名称:


Bulk Inquiry

Inquiry Information

蛋白 A/G 磁珠