2. Academic Validation
  3. The antifungal potential of (Z)-ligustilide and the protective effect of eugenol demonstrated by a chemometric approach

The antifungal potential of (Z)-ligustilide and the protective effect of eugenol demonstrated by a chemometric approach

  • Sci Rep. 2019 Jun 19;9(1):8729. doi: 10.1038/s41598-019-45222-y.
Alice M S Rodrigues 1,  Véronique Eparvier 2,  Guillaume Odonne 3,  Nadine Amusant 4,  Didier Stien 5,  Emeline Houël 6
Affiliations

Affiliations

  • 1 Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologie Microbienne, LBBM, Observatoire Océanologique, 66650, Banyuls-sur-mer, France.
  • 2 CNRS, Institut de Chimie des Substances Naturelles, UPR2301, Université Paris-Saclay, 91198, Gif-sur-Yvette, France.
  • 3 Laboratoire Ecologie, Evolution, Interactions des Systèmes Amazoniens (LEEISA), CNRS, Université de Guyane, IFREMER, 97300, Cayenne, France.
  • 4 CIRAD, UMR EcoFoG, AgroParisTech, CNRS, INRA, Université des Antilles, Université de Guyane, 97300, Cayenne, France.
  • 5 Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologie Microbienne, LBBM, Observatoire Océanologique, 66650, Banyuls-sur-mer, France. didier.stien@cnrs.fr.
  • 6 CNRS, UMR EcoFoG, AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, 97300, Cayenne, France. emeline.houel@ecofog.gf.
PMID: 31217530 DOI: 10.1038/s41598-019-45222-y
Abstract

Mankind is on the verge of a postantibiotic era. New concepts are needed in our battle to attenuate infectious diseases around the world and broad spectrum plant-inspired synergistic pharmaceutical preparations should find their place in the global fight against pathogenic Microorganisms. To progress towards the discovery of potent Antifungal agents against human pathologies, we embarked upon developing chemometric approach coupled with statistical design to unravel the origin of the anticandidal potential of a set of 66 essential oils (EOs). EOs were analyzed by GC-MS and tested against Candida albicans and C. parapsilosis (Minimal Inhibitory Concentration, MIC). An Orthogonal Partial Least Square (OPLS) analysis allowed us to identify six molecules presumably responsible for the anticandidal activity of the oils: (Z)-ligustilide, eugenol, eugenyl acetate, citral, thymol, and β-citronellol. These compounds were combined following a full factorial experimental design approach in order to optimize the anticandidal activity and selectivity index (SI = IC50(MRC5 cells)/MIC) through reconstituted mixtures. (Z)-Ligustilide and citral were the most active compounds, while (Z)-ligustilide and eugenol were the two main factors that most contributed to the increase of the SI. These two terpenes can, therefore, be used to construct bioinspired synergistic anticandidal mixtures.

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