2. Academic Validation
  3. Ac-YVAD-cmk improves neurological function by inhibiting caspase-1-mediated inflammatory response in the intracerebral hemorrhage of rats

Ac-YVAD-cmk improves neurological function by inhibiting caspase-1-mediated inflammatory response in the intracerebral hemorrhage of rats

  • Int Immunopharmacol. 2019 Oct;75:105771. doi: 10.1016/j.intimp.2019.105771.
Hongsheng Liang 1,  Yong Sun 2,  Aili Gao 3,  Nannan Zhang 4,  Ying Jia 5,  Shanshan Yang 6,  Meng Na 1,  Huailei Liu 1,  Xingbo Cheng 1,  Xiaofeng Fang 1,  Wei Ma 1,  Xiangtong Zhang 7,  Fan Wang 8
Affiliations

Affiliations

  • 1 Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, P.R. China.
  • 2 Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, P.R. China; Department of Neurosurgery, Xinyang Central Hospital, Xinyang, P.R. China.
  • 3 School of Life Science, Northeast Agricultural University, Harbin, P.R. China.
  • 4 Department of Epidemiology, School of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P.R. China.
  • 5 Department of Pathology, First Affiliated Hospital of Harbin Medical University, Harbin, P.R. China.
  • 6 Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, P.R. China.
  • 7 Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, P.R. China. Electronic address: zgxgtg@sina.com.
  • 8 Department of Epidemiology, School of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P.R. China. Electronic address: yifan.701@163.com.
PMID: 31352322 DOI: 10.1016/j.intimp.2019.105771
Abstract

Objective: Intracerebral hemorrhage (ICH) is acknowledged as a serious clinical problem lacking effective treatments. And caspase-1-mediated inflammatory response happened during the progression of ICH. Therefore, we aimed to investigate the effects of Caspase-1 inhibitor Ac-YVAD-cmk on ICH.

Materials and methods: Microglia cells were isolated and activated by Thrombin for 24 h. Then the transcript and protein expressions of NLRP3 and inflammatory factors were assessed by RT-PCR and western blotting. Moreover, Ac-YVAD-cmk was injected into the ICH model. The mNSS and brain water content were tested at 24 h post-ICH. Finally, the pathological changes of microglia activation following ICH were discovered by the immunohistochemical and HE staining ways.

Results: Ac-YVAD-cmk inhibited the activation of pro-caspase-1 and decreased brain edema, in association with decreasing activated microglia and the expression of inflammation-related factors at 24 h post-ICH. Consequently, Ac-YVAD-cmk reduced the release of mature IL-1β/IL-18 in perihematoma, improved the behavioral performance, and alleviated microglia in perihematoma region in ICH rats.

Conclusions: These results indicate that Caspase-1 could amplify the plural inflammatory responses in the ICH. Administration of Ac-YVAD-cmk has the potential to be a novel therapeutic strategy for ICH.

Keywords

Ac-YVAD-cmk; Caspase-1; Intracerebral hemorrhage; Microglia.

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