1. Academic Validation
  2. Exogenous IL-19 mediates downregulation of TGF-β through Erk and p38 pathway to inhibit epidural fibrosis

Exogenous IL-19 mediates downregulation of TGF-β through Erk and p38 pathway to inhibit epidural fibrosis

  • Eur Rev Med Pharmacol Sci. 2019 Sep;23(17):7184-7190. doi: 10.26355/eurrev_201909_18819.
Q-J Wang 1 A L Zhang Z-Q Kang Z-T Zhang Y-S Wang
Affiliations

Affiliation

  • 1 Department of Orthopedics, No. 89 Hospital of Chinese People's Liberation Army, Weifang, China. 215328430@qq.com.
Abstract

Objective: To evaluate the effect of interleukin-19 (IL-19) treatment on epidural fibrosis and its mechanism of action with transforming growth factor β (TGF-β).

Materials and methods: Initially, IL-19 (10, 20, 50 and 100 ng/L) was used to pretreat rat fibroblasts. TGF-β (10 μg/L) was then applied to activate fibroblasts. The protein expression levels of TGF-β Receptor, extracellular-signal-regulated kinase (ERK) and p-38 were measured by Western blotting. In addition, we performed laminectomy at T10 vertebral plate in rats, followed by injection of IL-19 in caudal vein one week after injury. Furthermore, IL-19, TGF-β and fibrosis indexes were measured by quantitative Real-time polymerase chain reaction (qRT-PCR) and Western blotting at 7 and 28 days after injury, respectively.

Results: Concentration-dependent IL-19 significantly down-regulated TGF-β Receptor expression and inhibited phosphorylated ERK (p-Erk) and phosphorylated p38 (p-p38). In vivo, IL-19 reduced the expressions of TGF-β and connective tissue growth factor (CTGF) at 7 days. Furthermore, IL-19 significantly suppressed extracellular matrix productions formation, including α smooth muscle actin (α-SMA) and collagen-1 (COL-1), and fibronectin at 28 days.

Conclusions: IL-19 inhibited TGF-β expression via ERK and p38 pathway. Moreover, it decreased CTGF expression to suppress α-SMA, COL-1 and fibronectin in scar tissues, thereby preventing spinal cord from compression of scar tissues.

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