2. Academic Validation
  3. Asatone and Isoasatone A Against Spodoptera litura Fab. by Acting on Cytochrome P450 Monoxygenases and Glutathione Transferases

Asatone and Isoasatone A Against Spodoptera litura Fab. by Acting on Cytochrome P450 Monoxygenases and Glutathione Transferases

  • Molecules. 2019 Oct 31;24(21):3940. doi: 10.3390/molecules24213940.
Ruimei Ling 1 2 3 4,  Renyue Yang 5 6 7 8,  Ping Li 9 10 11 12,  Xiongfei Zhang 13 14 15 16,  Tunkai Shen 17,  Xiaowen Li 18,  Quan Yang 19,  Lirong Sun 20,  Jian Yan 21 22 23 24
Affiliations

Affiliations

  • 1 Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China. ruimeiling0725@163.com.
  • 2 Guangdong Provincial Key Laboratory of Eco-Circular Agriculture, South China Agricultural University, Guangzhou 510642, China. ruimeiling0725@163.com.
  • 3 Guangdong Engineering Research Centre for Modern Eco-Agriculture, South China Agricultural University, Guangzhou 510642, China. ruimeiling0725@163.com.
  • 4 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China. ruimeiling0725@163.com.
  • 5 Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China. renyueyang123@126.com.
  • 6 Guangdong Provincial Key Laboratory of Eco-Circular Agriculture, South China Agricultural University, Guangzhou 510642, China. renyueyang123@126.com.
  • 7 Guangdong Engineering Research Centre for Modern Eco-Agriculture, South China Agricultural University, Guangzhou 510642, China. renyueyang123@126.com.
  • 8 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China. renyueyang123@126.com.
  • 9 Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China. liping2016@scau.edu.cn.
  • 10 Guangdong Provincial Key Laboratory of Eco-Circular Agriculture, South China Agricultural University, Guangzhou 510642, China. liping2016@scau.edu.cn.
  • 11 Guangdong Engineering Research Centre for Modern Eco-Agriculture, South China Agricultural University, Guangzhou 510642, China. liping2016@scau.edu.cn.
  • 12 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China. liping2016@scau.edu.cn.
  • 13 Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China. zhangfeiswymail@163.com.
  • 14 Guangdong Provincial Key Laboratory of Eco-Circular Agriculture, South China Agricultural University, Guangzhou 510642, China. zhangfeiswymail@163.com.
  • 15 Guangdong Engineering Research Centre for Modern Eco-Agriculture, South China Agricultural University, Guangzhou 510642, China. zhangfeiswymail@163.com.
  • 16 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China. zhangfeiswymail@163.com.
  • 17 Key Laboratory of State Administration of Traditional Chinese Medicine for Production & Development of Cantonese Medicinal Materials, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China. shentkcixi@163.com.
  • 18 School of Basic Medical Sciences and Key Laboratory of Mental Health of the Ministry of Education, Department of Neurobiology, Southern Medical University, Guangzhou 510642, China. lixw@smu.edu.cn.
  • 19 Key Laboratory of State Administration of Traditional Chinese Medicine for Production & Development of Cantonese Medicinal Materials, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China. yangquan7208@vip.163.com.
  • 20 School of Basic Medical Sciences and Key Laboratory of Mental Health of the Ministry of Education, Department of Neurobiology, Southern Medical University, Guangzhou 510642, China. slr0807@fimmu.com.
  • 21 Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China. yanjian78@scau.edu.cn.
  • 22 Guangdong Provincial Key Laboratory of Eco-Circular Agriculture, South China Agricultural University, Guangzhou 510642, China. yanjian78@scau.edu.cn.
  • 23 Guangdong Engineering Research Centre for Modern Eco-Agriculture, South China Agricultural University, Guangzhou 510642, China. yanjian78@scau.edu.cn.
  • 24 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China. yanjian78@scau.edu.cn.
PMID: 31683670 DOI: 10.3390/molecules24213940
Abstract

Asatone and isoasatone A from Asarum ichangense Cheng were determined to be defensive compounds to some insects in a previous investigation. However, the anti-insect activity mechanisms to caterpillar are still unclear. The compounds asatone and isoasatone A from A. ichangense were induced by Spodoptera litura. The anti-insect activity of asatone and isoasatone A to S. litura was further tested by weight growth rate of the insect through a diet experiment. Isoasatone A showed a more significant inhibitory effect on S. litura than asatone on the second day. The concentration of asatone was higher than isoasatone A in the second instar larvae of S. litura after 12 h on the feeding test diet. Both compounds caused mid-gut structural deformation and tissue decay as determined by mid-gut histopathology of S. litura. Furthermore, some detoxification Enzyme activity were measured by relative expression levels of genes using a qPCR detecting system. Asatone inhibited the gene expression of the Cytochrome P450 monooxygenases (P450s) CYP6AB14. Isoasatone A inhibited the relative expression levels of CYP321B1, CYP321A7, CYP6B47, CYP6AB14, and CYP9A39. Asatone increased the relative gene expression of the glutathione transferases (GSTs) SIGSTe1 and SIGSTo1, in contrast, isoasatone A decreased the relative gene expression of SIGSTe1 by about 33 fold. Neither compound showed an effect on acetylcholinesterase SIAce1 and SIAce2. The mechanism of anti-insect activity by both compounds could be explained by the inhibition of enzymes P450s and GSTs. The results provide new insights into the function of unique secondary metabolites asatone and isoasatone A in genus Asarum, and a new understanding of why A. ichangense is largely free of insect pests.

Keywords

Asarum ichangense; Spodoptera litura; anti-insect; asatone; induced defenses; isoasatone A.

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