1. Academic Validation
  2. Recombinant OX40 attenuates neuronal apoptosis through OX40-OX40L/PI3K/AKT signaling pathway following subarachnoid hemorrhage in rats

Recombinant OX40 attenuates neuronal apoptosis through OX40-OX40L/PI3K/AKT signaling pathway following subarachnoid hemorrhage in rats

  • Exp Neurol. 2020 Apr;326:113179. doi: 10.1016/j.expneurol.2020.113179.
Ling-Yun Wu 1 Budbazar Enkhjargal 2 Zhi-Yi Xie 2 Zachary D Travis 2 Cheng-Mei Sun 2 Ke-Ren Zhou 2 Tong-Yu Zhang 2 Qi-Quan Zhu 2 Chun-Hua Hang 3 John H Zhang 4
Affiliations

Affiliations

  • 1 Department of Neurosurgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China; Department of Physiology and Pharmacology, School of Medicine, Loma Linda University, Loma Linda, CA 92354, United States.
  • 2 Department of Physiology and Pharmacology, School of Medicine, Loma Linda University, Loma Linda, CA 92354, United States.
  • 3 Department of Neurosurgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China. Electronic address: hang_neurosurgery@163.com.
  • 4 Department of Physiology and Pharmacology, School of Medicine, Loma Linda University, Loma Linda, CA 92354, United States; Department of Physiology and Pharmacology, Department of Anesthesiology and Department of Neurosurgery, School of Medicine, Loma Linda University, Loma Linda, CA 92354, United States. Electronic address: johnzhang3910@yahoo.com.
Abstract

Subarachnoid hemorrhage (SAH) is the most devastating form of stroke. Reducing neuronal Apoptosis is an important countermeasure against early brain injury (EBI) after SAH. Recent evidence indicates that OX40-OX40L coupling is critical for cell survival and proliferation. Current study was performed to detect the role of recombinant OX40 (ReOX40) against neuronal Apoptosis after SAH. The endovascular perforation model of SAH was performed on Sprague-Dawley (SD) rats. ReOX40 was injected intracerebroventricularly (i.c.v) 1 h after SAH induction and the following methods were employed: neurological function evaluation, immunofluorescence staining, fluoro-Jade C staining, and western blot. To study the underlying precise molecular mechanism, small interfering ribonucleic acid (siRNA) for OX40L and a specific inhibitor of PI3K, LY294002, were injected i.c.v. into SAH + ReOX40 rats before induction of SAH. When compared with sham rats, the expression of OX40 and OX40L was seen to decrease in the brain at 24 h after SAH induction. Administration of ReOX40 (5 μg/kg) increased expression of the OX40L, reduced the neuronal Apoptosis, and improved short and long-term neurological function deficits. Furthermore, ReOx40 heightened activation of OX40L/PI3K/Akt axis, increased the downstream anti-apoptotic protein (Bcl2, Bcl-xL), and depressed the apoptotic protein (cleaved Caspase 3, Bax). However, the protective effects of ReOX40 were abolished by the administration of OX40L siRNA and LY294002, respectively. These results demonstrate that ReOX40 attenuates neuronal Apoptosis through OX40-OX40L/PI3K/Akt pathway in EBI after SAH.

Keywords

Early brain injury; Neuronal apoptosis; OX40; OX40L; Subarachnoid hemorrhage.

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