1. Academic Validation
  2. Tanshinone IIA Ameliorates Inflammation Response in Osteoarthritis via Inhibition of miR-155/FOXO3 Axis

Tanshinone IIA Ameliorates Inflammation Response in Osteoarthritis via Inhibition of miR-155/FOXO3 Axis

  • Pharmacology. 2021;106(1-2):20-28. doi: 10.1159/000505493.
Biao Zhou 1 2 Lin-Hui Li 1 Li-Ming Tan 3 4 Wen-Bing Luo 5 6 Hui Xiong 6 Xiao-Long Lu 7 Dan Liu 8 Wang-Yang Li 6 Yu-Xing Guo 6 Zhi Tang 6 Li-Guo Zhu 9
Affiliations

Affiliations

  • 1 Department of Orthopedics, Wangjing Hospital of Chinese Academy of Chinese Medical Science, Beijing, China.
  • 2 Department of Orthopedics, Xiangtan Hospital Affiliated to Nanhua University, Xiangtan, China.
  • 3 Department of Orthopedics, The Fourth Hospital of Changsha, Changsha, China.
  • 4 Department of Orthopedics, Changsha Hospital of Tranditional Chinese Medicine, Changsha, China.
  • 5 Department of Orthopedics, The Chinese Medicine Hospital of Linli County, Linli, China.
  • 6 Department of Orthopedics, Hunan University of Chinese Medicine, Changsha, China.
  • 7 Department of Orthopedics, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, China.
  • 8 Department of Rheumatology, The First Hospital of Hunan University of Chinese Medicine, Changsha, China.
  • 9 Department of Orthopedics, Wangjing Hospital of Chinese Academy of Chinese Medical Science, Beijing, China, zhuliguow712@163.com.
Abstract

Background: Osteoarthritis (OA) is the most common joint disorder characterized by degeneration of the articular cartilage and joint destruction with an associated risk of mobility disability in elderly people. Although a lot of achievements have been made, OA is still regarded as an incurable disease. Therefore, the pathological mechanisms and novel therapeutic strategies of OA need more investigation.

Methods: MTT assay was conducted to measure the viability of chondrocytes after LPS treatment. Cell Apoptosis was analyzed by annexin V/propidium iodide labeling. ELISA was used to determine the concentrations of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in the culture supernatant of chondrocytes. The expression level of miR-155, IL-1β, FOXO3, TNF-α, IL-6, Caspase-3, and caspase-9 in chondrocytes was analyzed by RT-qPCR or Western blot.

Results: We found that LPS led to inflammatory responses, cell Apoptosis, and increased miR-155 expression in human articular chondrocytes. Tanshinone IIA could inhibit LPS-induced inflammation and cell Apoptosis of chondrocytes via regulating the expression of miR-155 and FOXO3. miR-155 directly targeted the 3'-UTR of FOXO3 to regulate its expression.

Conclusions: Taken together, our data suggest tanshinone IIA ameliorates inflammation response in OA via inhibition of the miR-155/FOXO3 axis, and provide some evidences that tanshinone IIA could be designed and developed as a new promising clinical therapeutic drug for OA patients.

Keywords

FOXO3; Inflammation; Osteoarthritis; Tanshinone IIA; miR-155.

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