1. Academic Validation
  2. Tetramethylpyrazine inhibits proliferation of colon cancer cells in vitro

Tetramethylpyrazine inhibits proliferation of colon cancer cells in vitro

  • World J Clin Cases. 2021 Jun 26;9(18):4542-4552. doi: 10.12998/wjcc.v9.i18.4542.
Hua Li 1 Yan-Xu Hou 1 Yu Yang 2 Qing-Qiang He 1 Tian-Hua Gao 1 Xiao-Feng Zhao 1 Zhi-Bin Huo 1 Shu-Bo Chen 1 Deng-Xiang Liu 3
Affiliations

Affiliations

  • 1 Institute of Cancer Control, Xingtai People's Hospital, Xingtai 054001, Hebei Province, China.
  • 2 School of First Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China.
  • 3 Institute of Cancer Control, Xingtai People's Hospital, Xingtai 054001, Hebei Province, China. dengxianglfangliao@163.com.
Abstract

Background: Colon Cancer is one of the most common malignancies worldwide, and chemotherapy is a widely used strategy in colon Cancer clinical therapy. However, chemotherapy resistance is a major cause of disease recurrence and progression in colon Cancer, and thus novel drugs for treatment are urgently needed. Tetramethylpyrazine (TMP), a component of the traditional Chinese medicine Chuanxiong Hort, has been proven to exhibit a beneficial effect in tumors.

Aim: To investigate the potential Anticancer activity of TMP in colon Cancer and its underlying mechanisms.

Methods: Colon Cancer cells were incubated with different concentrations of TMP. Cell viability was evaluated by crystal violet staining assay and cell counting kit-8 assay, and cell Apoptosis and cell cycle were assessed by flow cytometry.

Results: TMP significantly inhibited the proliferation of colon Cancer cells in a dose- and time-dependent manner. In addition, flow cytometry revealed that TMP induced cell cycle arrest at the G0/G1 phase. TMP treatment caused early stage Apoptosis in SW480 cells, whereas it caused late stage Apoptosis in HCT116 cells.

Conclusion: Our studies demonstrated that TMP inhibits the proliferation of colon Cancer cells in a dose- and time-dependent manner by inducing Apoptosis and arresting the cell cycle at the G0/G1 phase. Our findings suggest that TMP might serve as a potential novel therapeutic drug in the treatment of human colon Cancer.

Keywords

Apoptosis; Cell cycle; Cell proliferation; Chemotherapy; Colon cancer; Tetramethylpyrazine.

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