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  2. Arabidopsis P4 ATPase-mediated cell detoxification confers resistance to Fusarium graminearum and Verticillium dahliae

Arabidopsis P4 ATPase-mediated cell detoxification confers resistance to Fusarium graminearum and Verticillium dahliae

  • Nat Commun. 2021 Nov 5;12(1):6426. doi: 10.1038/s41467-021-26727-5.
Fanlong Wang 1 Xianbi Li 1 Yujie Li 1 Jing Han 1 Yang Chen 1 Jianyan Zeng 1 Mei Su 1 Jingxin Zhuo 1 Hui Ren 1 Haoru Liu 1 Lei Hou 1 Yanhua Fan 1 Xingying Yan 1 Shuiqing Song 1 Juan Zhao 1 Dan Jin 1 Mi Zhang 1 Yan Pei 2
Affiliations

Affiliations

  • 1 The Affiliation Biotechnology Research Center, Southwest University, No. 2 Tiansheng Rd, Beibei, Chongqing, 400716, P. R. China.
  • 2 The Affiliation Biotechnology Research Center, Southwest University, No. 2 Tiansheng Rd, Beibei, Chongqing, 400716, P. R. China. peiyan3@swu.edu.cn.
Abstract

Many toxic secondary metabolites produced by phytopathogens can subvert host immunity, and some of them are recognized as pathogenicity factors. Fusarium head blight and Verticillium wilt are destructive plant diseases worldwide. Using toxins produced by the causal fungi Fusarium graminearum and Verticillium dahliae as screening agents, here we show that the Arabidopsis P4 ATPases AtALA1 and AtALA7 are responsible for cellular detoxification of mycotoxins. Through AtALA1-/AtALA7-mediated vesicle transport, toxins are sequestered in vacuoles for degradation. Overexpression of AtALA1 and AtALA7 significantly increases the resistance of transgenic Plants to F. graminearum and V. dahliae, respectively. Notably, the concentration of deoxynivalenol, a mycotoxin harmful to the health of humans and Animals, was decreased in transgenic Arabidopsis siliques and maize seeds. This vesicle-mediated cell detoxification process provides a strategy to increase plant resistance against different toxin-associated diseases and to reduce the mycotoxin contamination in food and feed.

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