1. Academic Validation
  2. SNHG3 could promote prostate cancer progression through reducing methionine dependence of PCa cells

SNHG3 could promote prostate cancer progression through reducing methionine dependence of PCa cells

  • Cell Mol Biol Lett. 2022 Feb 5;27(1):13. doi: 10.1186/s11658-022-00313-z.
Xiaotian Wang 1 Yongsheng Song 1 Yaxing Shi 1 Da Yang 1 Jiaxing Li 1 Bo Yin 2
Affiliations

Affiliations

  • 1 Department of Urology, Shengjing Hospital of China Medical University, No.36 Sanhao Street, Heping District, Shenyang, 110001, Liaoning, China.
  • 2 Department of Urology, Shengjing Hospital of China Medical University, No.36 Sanhao Street, Heping District, Shenyang, 110001, Liaoning, China. yinbo19751003@hotmail.com.
Abstract

In recent years, morbidity and mortality of prostate Cancer (PCa) have increased dramatically, while mechanistic understanding of its onset and progression remains unmet. LncRNA SNHG3 has been proved to stimulate malignant progression of multiple cancers, whereas its functional mechanism in PCa needs to be deciphered. In this study, our analysis in the TCGA database revealed high SNHG3 expression in PCa tissue. Further analysis in starBase, TargetScan, and mirDIP databases identified the SNHG3/miR-152-3p/SLC7A11 regulatory axis. FISH was conducted to assess the distribution of SNHG3 in PCa tissue. Dual-luciferase reporter gene and RIP assays confirmed the relationship among the three objects. Next, qRT-PCR and western blot were conducted to measure expression levels of SNHG3, miR-152-3p, and SLC7A11. CCK-8, colony formation, Transwell, and flow cytometry were carried out to assess proliferation, migration, invasion, methionine dependence, Apoptosis, and the cell cycle. It was noted that SNHG3 as a molecular Sponge of miR-152-3p stimulated proliferation, migration, and invasion, restrained methionine dependence and Apoptosis, and affected the cell cycle of PCa cells via targeting SLC7A11. Additionally, we constructed xenograft tumor models in nude mice and confirmed that knockdown of SNHG3 could restrain PCa tumor growth and elevate methionine dependence in vivo. In conclusion, our investigation improved understanding of the molecular mechanism of SNHG3 modulating PCa progression, thereby generating novel insights into clinical therapy for PCa.

Keywords

Methionine dependence; Prostate cancer; SLC7A11; SNHG3; miR-152-3p.

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