1. Academic Validation
  2. Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling

Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling

  • Cell Death Discov. 2022 Apr 11;8(1):190. doi: 10.1038/s41420-022-00975-w.
Zongwen Liang  # 1 Qiong Wu  # 1 Honglin Wang 1 Jiahuan Tan 1 Han Wang 1 Yanling Gou 1 Yingying Cao 1 Zhi Li 1 Zongfeng Zhang 2
Affiliations

Affiliations

  • 1 Department of Obstetrics and Gynecology, Second Affiliated Hospital of Harbin Medical University, 150086, Harbin, China.
  • 2 Department of Obstetrics and Gynecology, Second Affiliated Hospital of Harbin Medical University, 150086, Harbin, China. viaac1973@163.com.
  • # Contributed equally.
Abstract

Endometriosis is a chronic disorder characterized by the implantation of endometrial glands and stroma outside the uterus. However, the pathogenesis of endometriosis is still unclear. To date, there is no fully effective treatment without trauma because of various side effects. Recent data suggest that Ferroptosis is a novel recognized form of nonapoptosis-regulated cell death characterized by iron-dependent and lethal lipid peroxidation accumulation, showing great promise in the treatment of many diseases. In the present study, we verified that erastin induced Ferroptosis in ectopic endometrial stromal cells (EESCs). Furthermore, we found that the expression of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) was decreased during erastin-induced Ferroptosis. Knockdown of MALAT1 significantly aggravated the inhibition of cell viability and increased intracellular iron, Liperfluo, and MDA levels in EESCs upon erastin treatment. Mechanistically, we demonstrated that MALAT1 served as a competing endogenous RNA of miR-145-5p to regulate the expression of MUC1, a suppressor of Ferroptosis. MALAT1 knockdown-mediated ferroptotic cell death and MUC1 downregulation could be abrogated by inhibition of miR-145-5p. In addition, miR-145-5p inhibition-mediated ferroptotic cell death could be abolished by MUC1 knockdown. Furthermore, erastin-induced Ferroptosis shrunk endometriotic lesions via the MALAT1/miR-145-5p/MUC1 axis in vivo. Taken together, our data indicate that knockdown of MALAT1 facilitates Ferroptosis upon erastin treatment via miR-145-5p/MUC1 signaling. The synergistic effect of MALAT1 knockdown and erastin induction in Ferroptosis may be a new therapeutic strategy for endometriosis.

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