1. Academic Validation
  2. Contribution of RAS, ROS and COX-1-derived prostanoids to the contractile profile of perivascular adipose tissue in cafeteria diet-induced obesity

Contribution of RAS, ROS and COX-1-derived prostanoids to the contractile profile of perivascular adipose tissue in cafeteria diet-induced obesity

  • Life Sci. 2022 Sep 22;309:120994. doi: 10.1016/j.lfs.2022.120994.
Daniela Esteves Ferreira Dos Reis Costa 1 Natália Ferreira de Araújo 1 Natália Ribeiro Cabacinha Nóbrega 1 Naiara de Assis Rabelo Ribeiro 1 Amanda Carla Clemente de Oliveira 2 Luciano Dos Santos Aggum Capettini 3 Adaliene Versiani Matos Ferreira 2 Daniella Bonaventura 4
Affiliations

Affiliations

  • 1 Laboratory of Vascular Pharmacology, Department of Pharmacology, Biological Sciences Institute, Federal University of Minas Gerais, Brazil.
  • 2 Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais, Brazil.
  • 3 Laboratory of Vascular Biology, Department of Pharmacology, Biological Sciences Institute, Federal University of Minas Gerais, Brazil.
  • 4 Laboratory of Vascular Pharmacology, Department of Pharmacology, Biological Sciences Institute, Federal University of Minas Gerais, Brazil. Electronic address: danibona@icb.ufmg.br.
Abstract

Aims: Obesity can lead to the loss of the anticontractile properties of perivascular adipose tissue (PVAT). Given that cafeteria (CAF) diet reflects the variety of highly calorie and easily accessible foods in Western societies, contributing to obesity and metabolic disorders, we sought to investigate the impact of CAF diet on PVAT vasoactive profile and the involvement of renin-angiotensin system, oxidative stress, and cyclooxygenase pathway.

Main methods: Male Balb/c mice received standard or CAF diet for 4 weeks. Oral glucose tolerance and Insulin sensitivity tests were performed, and fasting serum glucose, Cholesterol and triglyceride parameters were determined. Vascular reactivity, fluorescence and immunofluorescence analyzes were carried out in intact thoracic aorta in the presence or absence of PVAT.

Key findings: CAF diet was effective in inducing obesity and metabolic disorders, as demonstrated by increased body weight gain and adiposity index, hyperlipidemia, hyperglycemia, glucose intolerance and Insulin insensitivity. Importantly, CAF diet led to a significant decrease in aortic contractility which was restored in the presence of PVAT, exhibiting therefore a contractile profile. The contractile effect of PVAT was associated with the activation of AT1 receptor, Reactive Oxygen Species, cyclooxygenase-1, thromboxane A2 and prostaglandin E2 receptors.

Significance: These findings suggest that the contractile profile of PVAT involving the renin-angiotensin system activation, Reactive Oxygen Species and cyclooxygenase-1 metabolites may be a protective compensatory adaptive response during early stage of CAF diet-induced obesity as an attempt to restore the impaired vascular contraction observed in the absence of PVAT, contributing to the maintenance of vascular tone.

Keywords

COX; Cafeteria diet; Obesity; PVAT; ROS; Renin-angiotensin system.

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