1. Academic Validation
  2. BMECs Ameliorate High Glucose-Induced Morphological Aberrations and Synaptic Dysfunction via VEGF-Mediated Modulation of Glucose Uptake in Cortical Neurons

BMECs Ameliorate High Glucose-Induced Morphological Aberrations and Synaptic Dysfunction via VEGF-Mediated Modulation of Glucose Uptake in Cortical Neurons

  • Cell Mol Neurobiol. 2023 Jul 7. doi: 10.1007/s10571-023-01366-0.
Yu-Qi Huang 1 2 Xiao Gu 1 2 Xiao Chen 1 2 Yi-Ting Du 1 2 Bin-Chi Chen 1 2 Feng-Yan Sun 3 4 5
Affiliations

Affiliations

  • 1 Department of Neurobiology and Research Institute for Aging and Medicine, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, 138 Yi-Xue-Yuan Road, Shanghai, 200032, People's Republic of China.
  • 2 National Clinical Research Center for Aging and Medicine, Shanghai Medical College, Fudan University, Shanghai, 200032, People's Republic of China.
  • 3 Department of Neurobiology and Research Institute for Aging and Medicine, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, 138 Yi-Xue-Yuan Road, Shanghai, 200032, People's Republic of China. fysun@shmu.edu.cn.
  • 4 National Clinical Research Center for Aging and Medicine, Shanghai Medical College, Fudan University, Shanghai, 200032, People's Republic of China. fysun@shmu.edu.cn.
  • 5 Shanghai Key Laboratory of Bioactive Small Molecules, School of Basic Medical Sciences, Institute of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, People's Republic of China. fysun@shmu.edu.cn.
Abstract

It has been demonstrated that diabetes cause neurite degeneration in the brain and cognitive impairment and neurovascular interactions are crucial for maintaining brain function. However, the role of vascular endothelial cells in neurite outgrowth and synaptic formation in diabetic brain is still unclear. Therefore, present study investigated effects of brain microvascular endothelial cells (BMECs) on high glucose (HG)-induced neuritic dystrophy using a coculture model of BMECs with neurons. Multiple immunofluorescence labelling and western blot analysis were used to detect neurite outgrowth and synapsis formation, and living cell imaging was used to detect uptake function of neuronal glucose transporters. We found cocultured with BMECs significantly reduced HG-induced inhibition of neurites outgrowth (including length and branch formation) and delayed presynaptic and postsynaptic development, as well as reduction of neuronal glucose uptake capacity, which was prevented by pre-treatment with SU1498, a vascular endothelial growth factor (VEGF) receptor antagonist. To analyse the possible mechanism, we collected BMECs cultured condition medium (B-CM) to treat the neurons under HG culture condition. The results showed that B-CM showed the same effects as BMEC on HG-treated neurons. Furthermore, we observed VEGF administration could ameliorate HG-induced neuronal morphology aberrations. Putting together, present results suggest that cerebral microvascular endothelial cells protect against hyperglycaemia-induced neuritic dystrophy and restorate neuronal glucose uptake capacity by activation of VEGF receptors and endothelial VEGF release. This result help us to understand important roles of neurovascular coupling in pathogenesis of diabetic brain, providing a new strategy to study therapy or prevention for diabetic dementia. Hyperglycaemia induced inhibition of neuronal glucose uptake and impaired to neuritic outgrowth and synaptogenesis. Cocultured with BMECs/B-CM and VEGF treatment protected HG-induced inhibition of glucose uptake and neuritic outgrowth and synaptogenesis, which was antagonized by blockade of VEGF receptors. Reduction of glucose uptake may further deteriorate impairment of neurites outgrowth and synaptogenesis.

Keywords

Brain microvascular endothelial cell; Diabetes; Glucose uptake; Neuritic dystrophy; Synapsis formation; VEGF.

Figures
Products