Nitrogenous chemical constituents and their antitumor activities evaluation in vitro from the aerial parts of Corydalis impatiens (pall.) Fisch

Four new compounds, impatienines E-H (1-4), together with 18 known ones (R)-N-methylcoclaurine (5), impatienine I (6), thalifoline (7), iseluxine (8), pisoquinoline (9), corydaldine (10), northalifoline (11), noroxyhydrastinine (12), 6,7-methylenedioxy-1(2H)-isoquinolinone (13), N-methylcorydaldine (14), oxyhydrastinine (15), corypalline (16), N-trans-feruloylmethoxytyramine (17), N-trans-feruloyldopamine (18), N-trans-feruloyltyramine (19), N-trans-sinapoyltyramine (20), N-cis-feruloyltyramine (21), N-cis-sinapoyltyramine (22) were obtained from the aerial parts of Corydalis impatiens (pall.) Fisch. Their structures were elucidated by extensive spectroscopic analysis (1D- and 2D-NMR, HR-ESIMS, IR, UV) and/or comparison with reported literature. The inhibitory effects of these isolates were also evaluated against the growth of Cancer cells (A549, H1299 and HepG2). Compounds 2 and 4 showed significant inhibitory effect on HepG2 Cancer cells with IC₅₀ values of 8.62, 8.32 μM, respectively (positive control cisplatin: IC₅₀, 6.32 μM). Compounds 22 and 4 exhibited moderate inhibitory effects against A549 Cancer cells, and the IC₅₀ values were 7.78 and 12.54 μM, respectively (positive control cisplatin: IC₅₀, 1.83 μM).

Corydalis impatiens (pall.) Fisch; Papaveraceae; antitumor activity; nitrogenous chemical constituent; structure elucidation.