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  3. Characterization of excitatory synaptic transmission in the retrosplenial cortex of adult mice

Characterization of excitatory synaptic transmission in the retrosplenial cortex of adult mice

  • Mol Pain. 2025 Jan-Dec:21:17448069251335500. doi: 10.1177/17448069251335500.
Jinjin Wan 1,  Yujie Ma 1,  Xuanying Chen 2,  Wucheng Tao 2,  Shun Hao 1 2,  Wujun Geng 1,  Yili Wu 1 3 4,  Min Zhuo 1 2 5 6
Affiliations

Affiliations

  • 1 Oujiang Laboratory (Zhejiang Laboratory for Regenerative Medicine, Vision and Brain Health), Wenzhou Medical University, Wenzhou, Zhejiang, China.
  • 2 Key Laboratory of Brain Aging and Neurodegenerative Diseases, Fujian Medical University, Fuzhou, Fujian, China.
  • 3 Wenzhou Key Laboratory of Basic and Translational Research for Mental Disorders, Zhejiang Provincial Clinical Research Center for Mental Health, Wenzhou Medical University, Wenzhou, Zhejiang, China.
  • 4 Key Laboratory of Alzheimer's Disease of Zhejiang Province, School of Mental Health and The Affiliated Kangning Hospital, Institute of Aging, Wenzhou Medical University, Wenzhou, Zhejiang, China.
  • 5 Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Brain Diseases, Qingdao University, Qingdao, Shandong, China.
  • 6 Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
PMID: 40317242 DOI: 10.1177/17448069251335500
Abstract

The retrosplenial cortex (RSC) plays an important role in navigation, memory and pain. However, there are few studies on excitatory synaptic transmission in the RSC. Here, we used a multi-electrode array recording system (MED64) to study the characteristics of excitatory synaptic transmission in the RSC and the contribution of different types of voltage-gated CA2+ channels (VGCCs) in excitatory synaptic transmission. We found that glutamate is the major excitatory transmitter for RSC, and postsynaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors contribute to excitatory synaptic transmission. We also found that the N-type calcium channel blocker ω-conotoxin GVIA (ω-Ctx GVIA) had an inhibitory effect on basal synaptic transmission. The inhibitory effect was not consistent across channels, suggesting the actions effect of N-type VGCCs in RSC was inhomogeneous in spatial distribution. Our findings provide strong evidence that excitatory synaptic transmission in the RSC is mainly mediated by AMPA receptors and that N-type VGCCs mediate fast synaptic transmission in the RSC of adult mice.

Keywords

AMPA receptor; Retrosplenial cortex; synaptic transmission; voltage-gated calcium channels; ω-conotoxin GVIA.

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