2. Academic Validation
  3. Biotransformation and activity identification of icariin in human intestinal fungus Pichia occidentalis

Biotransformation and activity identification of icariin in human intestinal fungus Pichia occidentalis

  • J Asian Nat Prod Res. 2025 May 21:1-12. doi: 10.1080/10286020.2025.2493937.
Jia-Shuo Li 1,  Ru-Xin Yao 2,  Qing Lv 3,  Gang Wang 1,  Xue Wang 2,  Yu-Fei Wang 4,  Fang-Yu Yang 4,  Guo-Biao Liang 4,  Zhuang Miao 5
Affiliations

Affiliations

  • 1 Graduate School, Dalian Medical University, Dalian116000, China.
  • 2 School of Pharmacy, Dalian Medical University, Dalian116000, China.
  • 3 Laboratory Animal Center, Dalian Medical University, Dalian116000, China.
  • 4 Institute of Neurology, General Hospital of Shenyang Military Command, Shenyang110000, China.
  • 5 Anesthesiology, The First Affiliated Hospital of Dalian Medical University, Dalian116000, China.
PMID: 40396926 DOI: 10.1080/10286020.2025.2493937
Abstract

Compared to icariin, icariside II demonstrates superior absorption and metabolic properties along with enhanced bioactivity. In this study, using DXM-Glc fluorescence assay, Pichia occidentalis was identified as an intestinal fungus expressing functional glucosidase, which could efficiently convert icariin to icariside II via glycosidic bond hydrolysis, revealing the fungus-mediated intestinal glycoside metabolism pathway and determining the optimal conditions for the conversion, including temperature, drug loading, pH and reaction time. Meanwhile, icariside II exhibited anti-glioma activity, and its mechanism of action involved the inhibition of the activation of NF-κB signaling pathway in glioma cells.

Keywords

Biotransformation; Pichia occidentalis; anti-tumour; fungi; icariside II.

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