2. Academic Validation
  3. Aberrant downregulation of Y-box binding protein 1 expression impairs the cell cycle in an m5C-dependent manner in human granulosa cells from patients with primary ovarian insufficiency

Aberrant downregulation of Y-box binding protein 1 expression impairs the cell cycle in an m5C-dependent manner in human granulosa cells from patients with primary ovarian insufficiency

  • Cell Mol Life Sci. 2025 May 21;82(1):206. doi: 10.1007/s00018-025-05709-6.
Qichao Chen # 1 2,  Sisi Wang # 1 2,  Min Zhang # 1 2,  Yu Xiang 1 2,  Qingqing Chen 1 2,  Zhekun Li 1 2,  Yang Song 1 2,  Long Bai # 3 4 5,  Yimin Zhu # 6 7 8 9
Affiliations

Affiliations

  • 1 Key Laboratory of Reproductive Genetics (Ministry of Education), Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310002, Zhejiang, China.
  • 2 Department of Reproductive Endocrinology, School of Medicine, Women's Hospital, Zhejiang University, Hangzhou, 310002, Zhejiang, China.
  • 3 Key Laboratory of Reproductive Genetics (Ministry of Education), Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310002, Zhejiang, China. bailong0375@zju.edu.cn.
  • 4 Department of Reproductive Endocrinology, School of Medicine, Women's Hospital, Zhejiang University, Hangzhou, 310002, Zhejiang, China. bailong0375@zju.edu.cn.
  • 5 Zhejiang Key Laboratory of Maternal and Infant Health, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310002, Zhejiang, China. bailong0375@zju.edu.cn.
  • 6 Key Laboratory of Reproductive Genetics (Ministry of Education), Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310002, Zhejiang, China. zhuyim@zju.edu.cn.
  • 7 Department of Reproductive Endocrinology, School of Medicine, Women's Hospital, Zhejiang University, Hangzhou, 310002, Zhejiang, China. zhuyim@zju.edu.cn.
  • 8 Zhejiang Key Laboratory of Maternal and Infant Health, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310002, Zhejiang, China. zhuyim@zju.edu.cn.
  • 9 Institute of Medical Genetics and Development, Zhejiang University, Hangzhou, 310002, Zhejiang, China. zhuyim@zju.edu.cn.
  • # Contributed equally.
PMID: 40397139 DOI: 10.1007/s00018-025-05709-6
Abstract

Y-box binding protein 1 (YBX1) has been reported to play a role in human granulosa cell (GC) dysfunction by binding with long noncoding RNAs in patients with primary ovarian insufficiency (POI). 5-Methylcytosine (m5C) methylation is an abundant RNA epigenetic modification that is widely present in eukaryotic RNAs. However, whether YBX1, an important m5C reader, whether YBX1 participates in POI in an m5C- dependent manner remains unknown. Here, we demonstrated that the expression levels of YBX1 were decreased in GCs from patients with biochemical POI. YBX1 knockdown in a human granulosa cell line (KGN) impaired cell proliferation by preventing the G1 to S transition in the cell cycle. Conversely, YBX1 overexpression promoted the KGN cell proliferation. Integrated analysis of the transcriptome and m5C methylome profiles revealed that in human GCs, knockdown of YBX1 expression destabilized cell cycle-associated transcripts in an m5C-dependent manner, resulting in cell cycle arrest. Our results provide new insights of the pathogenesis of POI, revealing an alternative molecular mechanism in which YBX1 participates in human GC dysfunction by affecting the stability of cell cycle-associated genes in an m5C-dependent manner and thereby modulating GC proliferation.

Keywords

Cell cycle; Granulosa cell; M5C; Primary ovarian insufficiency; YBX1.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z