2. Academic Validation
  3. Protocol for isolating extracellular vesicles from human or mouse airway macrophages for functional assays and in vivo or in vitro experimentation

Protocol for isolating extracellular vesicles from human or mouse airway macrophages for functional assays and in vivo or in vitro experimentation

  • STAR Protoc. 2025 Jun 20;6(2):103867. doi: 10.1016/j.xpro.2025.103867.
Ezgi Sari 1,  Robert P Richter 2,  Delores A Stacks 3,  Crystal Lewis 3,  Julian B Smith 4,  Ningyong Xu 4,  Dakota C Finley 4,  Kristopher R Genschmer 4,  Camilla Margaroli 5
Affiliations

Affiliations

  • 1 University of Alabama at Birmingham, Graduate Biomedical Sciences, Birmingham, AL 35233, USA; Department of Pathology, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, AL 35233, USA. Electronic address: esari@uab.edu.
  • 2 Department of Pediatrics, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, AL 35233, USA.
  • 3 Department of Pathology, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, AL 35233, USA.
  • 4 Department of Medicine, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, AL 35233, USA.
  • 5 Department of Pathology, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, AL 35233, USA. Electronic address: cmargaroli@uabmc.edu.
PMID: 40460201 DOI: 10.1016/j.xpro.2025.103867
Abstract

Macrophages are key players in host defense and lung injury, and their crosstalk with Other cells dictates the course of the inflammatory response and tissue remodeling. Here, we present a protocol for isolating extracellular vesicles (EVs) from human bronchoalveolar lavage fluid (BALF) and for generating EVs from primary human or murine alveolar macrophages following stimulation by cigarette smoke extract. Furthermore, we describe how to measure matrix metalloproteinase (MMP)-12 activity on macrophage-derived EVs using a Förster resonance energy transfer assay.

Keywords

Cell isolation; Health Sciences; Immunology.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z