2. Academic Validation
  3. Establishment and characterization of preclinical model of primary ovarian squamous cell carcinoma

Establishment and characterization of preclinical model of primary ovarian squamous cell carcinoma

  • Hum Cell. 2025 Jul 7;38(5):125. doi: 10.1007/s13577-025-01253-4.
Chenyang Xu # 1 2,  Weiming Xu # 1 3,  Conghui Wu 1 2,  Chen Xu 1 2,  Zhengxiao Ma 1 2,  Jiamin Wang 2,  Yue Zhuo 1 2,  Xinan Cai 2,  Yuting Zhang 1 2,  Yingqi Lyu 1,  Jinchao Wang 2,  Minran Huang 2,  Shu Sun 2,  Tingting Feng 2,  Lisha Ying 4 5,  Dan Su 6 7
Affiliations

Affiliations

  • 1 Postgraduate Training Base Alliance of Wenzhou Medical University (Zhejiang Cancer Hospital), Hangzhou, 310022, Zhejiang, China.
  • 2 Department of Pathology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, 310022, Zhejiang, China.
  • 3 Department of Pathology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai, 317000, Zhejiang Province, China.
  • 4 Postgraduate Training Base Alliance of Wenzhou Medical University (Zhejiang Cancer Hospital), Hangzhou, 310022, Zhejiang, China. yingls@zjcc.org.cn.
  • 5 Zhejiang Cancer Institute, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, 310022, Zhejiang, China. yingls@zjcc.org.cn.
  • 6 Postgraduate Training Base Alliance of Wenzhou Medical University (Zhejiang Cancer Hospital), Hangzhou, 310022, Zhejiang, China. sudan@zjcc.org.cn.
  • 7 Department of Pathology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, 310022, Zhejiang, China. sudan@zjcc.org.cn.
  • # Contributed equally.
PMID: 40624380 DOI: 10.1007/s13577-025-01253-4
Abstract

Primary ovarian squamous cell carcinoma (POSCC) represents an exceedingly rare subtype of epithelial ovarian Cancer (EOC) characterized by a cryptic etiology and insidious onset. The rarity and high mortality associated with pure primary ovarian squamous cell carcinoma (SCC) make it challenging to conduct large randomized controlled studies. Furthermore, there are currently no commercially available ovarian SCC cell lines for research purposes, necessitating the urgent establishment of novel lines. To our knowledge, this study reports the first preclinical model of primary ovarian squamous cell carcinoma (POSCC), denoted as ZOC254. We have detailed the establishment and characterization of ZOC254, derived from a 64-year-old female patient, which preserves the primary tumor's original traits across various levels during prolonged in vitro expansion. Whole-exome Sequencing (WES) of both the primary tumor and derived cell line revealed homologous recombination deficiency (HRD) and high tumor mutational burden (TMB). ZOC254 also exhibited the PIK3CA: p.E542K mutation associated with targeted therapy. The effectiveness of olaparib, everolimus, and conventional chemotherapeutic agents for ovarian Cancer was preliminarily assessed on the growth of patient-derived cells (PDC). The POSCC cell line and derived xenograft transplantation model reported in this study serve the purpose of broadening the resources accessible for preclinical investigations into ovarian squamous carcinoma.

Keywords

Cell line establishment; HRD; Ovarian squamous cell carcinoma; TMB; Targeted therapy.

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