2. Academic Validation
  3. Iron overload exaggerates renal ischemia-reperfusion injury by promoting tubular cuproptosis via interrupting function of LIAS

Iron overload exaggerates renal ischemia-reperfusion injury by promoting tubular cuproptosis via interrupting function of LIAS

  • Redox Biol. 2025 Aug 2:86:103795. doi: 10.1016/j.redox.2025.103795.
Siyue Chen 1,  Tingting Chen 2,  Cuidi Xu 3,  Xiaohan Yu 4,  Junyu Shi 5,  Cheng Yang 6,  Tongyu Zhu 7
Affiliations

Affiliations

  • 1 Department of Kidney Transplantation, Zhongshan Hospital, Fudan University, China; Shanghai Key Laboratory of Organ Transplantation, Shanghai, 200032, China. Electronic address: 17301050132@fudan.edu.cn.
  • 2 Shanghai Key Laboratory of Organ Transplantation, Shanghai, 200032, China; Department of Pharmacy, Zhongshan Hospital, Fudan University, Shanghai, China. Electronic address: chen.tingting2@zs-hospital.sh.cn.
  • 3 Department of Kidney Transplantation, Zhongshan Hospital, Fudan University, China; Shanghai Key Laboratory of Organ Transplantation, Shanghai, 200032, China. Electronic address: 18211280003@fudan.edu.cn.
  • 4 Department of Kidney Transplantation, Zhongshan Hospital, Fudan University, China; Shanghai Key Laboratory of Organ Transplantation, Shanghai, 200032, China. Electronic address: 15255996628@163.com.
  • 5 Department of Kidney Transplantation, Zhongshan Hospital, Fudan University, China; Shanghai Key Laboratory of Organ Transplantation, Shanghai, 200032, China. Electronic address: 19301050182@fudan.edu.cn.
  • 6 Department of Kidney Transplantation, Zhongshan Hospital, Fudan University, China; Shanghai Key Laboratory of Organ Transplantation, Shanghai, 200032, China; Zhangjiang Institue of Fudan University, Shanghai, China. Electronic address: esuperyc@163.com.
  • 7 Department of Kidney Transplantation, Zhongshan Hospital, Fudan University, China; Shanghai Key Laboratory of Organ Transplantation, Shanghai, 200032, China; Shanghai Medical Collage, Fudan University, Shanghai, China. Electronic address: tyzhu@fudan.edu.cn.
PMID: 40753758 DOI: 10.1016/j.redox.2025.103795
Abstract

Renal ischemia-reperfusion injury (RIRI), a major contributor to acute kidney injury (AKI) and delayed graft function (DGF), is closely associated with dysregulation of metal ion homeostasis. Although copper and iron metabolism exhibit interconnected regulatory pathways, the temporal dynamics and functional interplay of these metal ions in RIRI pathogenesis remain poorly understood. Our study demonstrates that Cuproptosis and Ferroptosis, two distinct forms of cell death induced by metal ion overload, occur simultaneously within 6 h after reperfusion. Notably, ischemia-reperfusion injury induced iron overload significantly sensitizes renal tubular cells to copper-mediated cytotoxicity. Mechanistic investigations demonstrate that hypoxia-reoxygenation triggers Fe (II) accumulation, which subsequently downregulates [4Fe-4S] cluster assembly proteins. This impairment directly compromises the structural integrity of the cuproptosis-regulating protein LIAS by inducing [4Fe-4S] cluster loss, ultimately leading to defective protein lipoylation that drives Cuproptosis progression. Crucially, these pathological effects can be attenuated through either overexpression of [4Fe-4S] cluster assembly machinery or therapeutic application of iron-chelating agents. Our findings establish a novel iron-copper crosstalk mechanism in RIRI pathophysiology and propose targeted strategies focusing on [4Fe-4S] cluster homeostasis and iron chelation for clinical intervention.

Keywords

Acute kidney injury (AKI); Copper cytotoxicity; Cuproptosis; Ferroptosis; Iron overload; Metal ion homeostasis; Protein lipoylation; Renal ischemia-reperfusion injury.

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