2. Academic Validation
  3. Oleic acid activates TGFβ-Smad3 signaling to promote ovarian cancer progression

Oleic acid activates TGFβ-Smad3 signaling to promote ovarian cancer progression

  • J Ovarian Res. 2025 Aug 11;18(1):180. doi: 10.1186/s13048-025-01763-7.
Zhengyang Guo # 1 2 3,  Yinjia Li # 1 2 3,  Yunyun Guo 2 4,  Aosong Zhang 1,  Xiao Huo 1 2 3,  Ying Song 1 2 3,  Bing Li 1,  Yuanjun Tang 1 2 3,  Tianhui He 5,  Tong Liu 6 7 8,  Lixiang Xue 9 10 11 12,  Yi Qu 13 14 15 16 17,  Jiagui Song 18 19 20
Affiliations

Affiliations

  • 1 Cancer Center of Peking University Third Hospital, Beijing, 100191, China.
  • 2 Center of Basic Medical Research, Institute of Medical Innovation and Research, Peking University Third Hospital, Beijing, 100191, China.
  • 3 Beijing Key Laboratory for Interdisciplinary Research in Gastrointestinal Oncology (BLGO), Beijing, 100191, China.
  • 4 Department of Radiation Oncology, Peking University Third Hospital Cancer Center, Peking University Third Hospital, Beijing, 100191, China.
  • 5 Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.
  • 6 Cancer Center of Peking University Third Hospital, Beijing, 100191, China. tongtongliu@bjmu.edu.cn.
  • 7 Center of Basic Medical Research, Institute of Medical Innovation and Research, Peking University Third Hospital, Beijing, 100191, China. tongtongliu@bjmu.edu.cn.
  • 8 Beijing Key Laboratory for Interdisciplinary Research in Gastrointestinal Oncology (BLGO), Beijing, 100191, China. tongtongliu@bjmu.edu.cn.
  • 9 Cancer Center of Peking University Third Hospital, Beijing, 100191, China. lixiangxue@hsc.pku.edu.cn.
  • 10 Center of Basic Medical Research, Institute of Medical Innovation and Research, Peking University Third Hospital, Beijing, 100191, China. lixiangxue@hsc.pku.edu.cn.
  • 11 Beijing Key Laboratory for Interdisciplinary Research in Gastrointestinal Oncology (BLGO), Beijing, 100191, China. lixiangxue@hsc.pku.edu.cn.
  • 12 Department of Radiation Oncology, Peking University Third Hospital Cancer Center, Peking University Third Hospital, Beijing, 100191, China. lixiangxue@hsc.pku.edu.cn.
  • 13 State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China. pku_quyi@163.com.
  • 14 National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital), Beijing, 100191, China. pku_quyi@163.com.
  • 15 Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, 100191, China. pku_quyi@163.com.
  • 16 Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China. pku_quyi@163.com.
  • 17 National Clinical Key Specialty Construction Program, Beijing, 100191, China. pku_quyi@163.com.
  • 18 Cancer Center of Peking University Third Hospital, Beijing, 100191, China. jiaguisong@bjmu.edu.cn.
  • 19 Center of Basic Medical Research, Institute of Medical Innovation and Research, Peking University Third Hospital, Beijing, 100191, China. jiaguisong@bjmu.edu.cn.
  • 20 Beijing Key Laboratory for Interdisciplinary Research in Gastrointestinal Oncology (BLGO), Beijing, 100191, China. jiaguisong@bjmu.edu.cn.
  • # Contributed equally.
PMID: 40790593 DOI: 10.1186/s13048-025-01763-7
Abstract

Background: Ovarian Cancer represents the most aggressive and lethal gynecological Cancer, frequently demonstrating a distinct propensity for abdominal metastasis. Malignant ascites caused by abdominal metastasis provide a tumor microenvironment (TME) in ovarian Cancer. Notably, oleic acid is abundant in ovarian Cancer ascites, though its functional significance in TME modulation and tumor metastatic regulation remains poorly characterized. Stearoyl-CoA desaturase 1 (SCD1) is the key enzyme in the synthesis of oleic acid. Our study systematically explores the pathological role of oleic acid and evaluates the therapeutic effects of SCD1 inhibitor in ovarian Cancer progression.

Results: Oleic acid treatment significantly enhanced proliferation of ovarian Cancer cells and patient-derived organoids. Remarkably, oleic acid also increased membrane fluidity and promoted cell migration. Mechanistically, TGFβ-Smad3 signaling cascade is selectively activated by oleic acid, and inhibited by SCD1 suppression. Importantly, activation of SMAD3 caused by oleic acid treatment triggered epithelial-mesenchymal transition of ovarian Cancer cells. Clinical relevance was established that SCD1 expression was positively correlated with the activity of SMAD3 in ovarian Cancer tissues. Finally, in vivo studies showed that SCD1 inhibitor treatment suppressed tumor progression during intraperitoneal dissemination.

Conclusion: This study provides novel insights into the supporting role of oleic acid in fueling tumor proliferation and metastasis, mechanistically associated with its specific activation of TGFβ-Smad3 signaling. Therapeutically, pharmacological targeting oleic acid synthesis by SCD1 inhibitor emerges as a promising strategy for precision oncology in ovarian Cancer management.

Keywords

Epithelial-mesenchymal transition; Oleic acid; Ovarian cancer; SCD1; TGFβ-Smad3 signaling.

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