14-3-3 sigma 蛋白, Mouse

14-3-3 sigma proteins are adapter proteins in various signaling pathways that bind to phosphoserine or phosphothreonine motifs to regulate chaperone activity. When bound to KRT17, it stimulates the regulation of protein synthesis and cell growth through the Akt/mTOR pathway. 14-3-3 sigma Protein, Mouse is the recombinant mouse-derived 14-3-3 sigma protein, expressed by E. coli , with tag free.

14-3-3 sigma 蛋白作为衔接子发挥作用，涉及广泛的一般和专门信号传导途径的调节，通过识别磷酸丝氨酸或磷酸苏氨酸基序与众多伙伴结合，从而调节结合伙伴的活性。特别是，当与 KRT17 结合时，它通过刺激 Akt/mTOR 途径发挥调节蛋白质合成和上皮细胞生长的作用。此外，它还可能调节 MDM2 自动泛素化和降解，从而导致 p53/TP53 的激活。14-3-3 sigma 以同二聚体形式存在，与 XPO7、EIF4A1、ARHGAP1、VPS26A、VPS29 和 VPS35 形成复合物，并与多种蛋白质相互作用，包括 GAB2、KRT17、SAMSN1、SRPK2、COPS6、COP1（导致蛋白酶体降解）、DAPK2、PI4KB、SLITRK1 和 LRRK2 的“Thr-369”磷酸化形式（依赖于 LRRK2 磷酸化）。这些不同的相互作用凸显了 14-3-3 sigma 在协调各种细胞过程和信号事件中的多功能作用。

Mouse

E. coli

Tag Free

O70456 (M1-S248)

55948  [NCBI]

Full Length

MERASLIQKAKLAEQAERYEDMAAFMKSAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVKEYREKVETELRGVCDTVLGLLDSHLIKGAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADSAGEEGGEAPEEPQS

Approximately 32 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.