4-1BB/TNFRSF9 蛋白, Mouse (HEK293, Fc, solution)

4-1BB (CD137; TNFRSF9), a receptor of TNFSF9/4-1BBL, belongs to the tumor necrosis factor (TNF) receptor superfamily^[1]. 4-1BB is helpful for T cell activation and development, and also induces peripheral mononuclear cell proliferation and migration to the tumor microenvironment^[2]. 4-1BB is also involved in enhancing Nrf2 and NF-κB pathway mediated apoptosis of endothelial cells^[3]. Mouse 4-1BB protein is a surface glycoprotein with a transmembrane domain (188-208 a.a.). 4-1BB/TNFRSF9 Protein, Mouse (HEK293, Fc, solution) is the extracellular part (V24-L187) of 4-1BB protein, produced by HEK293 cells with C-terminal Fc-tag.

4-1BB，由TNFRSF9（CD137，ILA）编码，属于肿瘤坏死因子（TNF）受体超家族。4-1BB是一种表面糖蛋白，在多种细胞中表达，例如T细胞、B细胞、自然杀伤（NK）细胞、树突状（DC）、嗜酸性粒细胞和肥大细胞；甚至多种肿瘤细胞如人类白血病细胞。广泛分布于血管平滑肌、肿瘤血管壁和肝细胞癌的肝组织中。4-1BB 优先选择 CD8+ 细胞，而不是 CD4+ 细胞。它提供共刺激信号并激活 CD8+ T 细胞的细胞毒性作用，并有助于形成记忆 T 细胞。最后，它促进免疫系统对抗肿瘤。此外，CD137 将 CD137L 与信号单核细胞结合，增加其存活、增殖并刺激其迁移和外渗。此外，它还诱导多种促炎因子的释放，导致炎症单核细胞涌入组织，形成炎症环境^[1]。具体来说，CD137通过上调Fra1的表达来促进单核细胞/巨噬细胞向肿瘤微环境的迁移。同时还促进单核细胞/巨噬细胞分化为破骨细胞，从而为乳腺癌细胞在骨中定植和生长提供有利的微环境。它为乳腺癌转移提供了一种有前景的治疗策略^[2]。此外，CD137 信号传导通过分别由 Nrf2 和 NF-κB 通路介导的促氧化和促炎症机制促进内皮细胞 (EC) 凋亡^[3]。4-1BB蛋白在人和小鼠体内的同源性较低，序列相似度为56.75%。

4-1BB (hamster ovary; 5 μg/mL; 16 h) costimulates B lymphocyte proliferation^[4].

Mouse

HEK293

C-Fc

P20334 (V24-L187)

21942  [NCBI]

VQNSCDNCQPGTFCRKYNPVCKSCPPSTFSSIGGQPNCNICRVCAGYFRFKKFCSSTHNAECECIEGFHCLGPQCTRCEKDCRPGQELTKQGCKTCSLGTFNDQNGTGVCRPWTNCSLDGRSVLKTGTTEKDVVCGPPVVSFSPSTTISVTPEGGPGGHSLQVL

60-70 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Ye L, et al. CD137, an attractive candidate for the immunotherapy of lung cancer. Cancer Sci. 2020 May;111(5):1461-1467.  [Content Brief]

  [2]. Dass S Vinay, et al. 4-1BB (CD137), an Inducible Costimulatory Receptor, as a Specific Target for Cancer Therapy. BMB Rep. 2014 Mar;47(3):122-9.  [Content Brief]

  [3]. Jiang P, et al. CD137 promotes bone metastasis of breast cancer by enhancing the migration and osteoclast differentiation of monocytes/macrophages. Theranostics. 2019 May 9;9(10):2950-2966.  [Content Brief]

  [4]. Geng T, et al. CD137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating NF-κB Pathway. Mediators Inflamm. 2020 Jun 6;2020:4321912.  [Content Brief]

  [5]. Pauly S, et al. CD137 is expressed by follicular dendritic cells and costimulates B lymphocyte activation in germinal centers. J Leukoc Biol. 2002 Jul;72(1):35-42.  [Content Brief]