2. 重组蛋白
  3. Enzymes & Regulators
  4. Ligases (EC 6)
  5. AARS1 蛋白, Mouse (sf9, His, solution)

AARS1 蛋白, Mouse (sf9, His, solution)

目录号: HY-P75567Y
COA 产品使用指南 技术支持

AARS1蛋白促进丙氨酸附着到tRNA(Ala)上的两步过程：首先，丙氨酸被ATP激活形成Ala-AMP，然后转移到tRNA(Ala)的受体端。AARS1 还通过其编辑域纠正带错误电荷的 tRNA(Ala)。AARS1 蛋白, Mouse (sf9, His, solution) 是重组的 AARS1 蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。

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规格 价格 是否有货 数量
50 μg ￥2650 In-stock
100 μg ￥4500 In-stock
> 100 μg   询价  

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描述

The AARS1 protein facilitates the two-step process of attaching alanine to tRNA(Ala): first, alanine is activated by ATP to form Ala-AMP, and then it is transferred to the acceptor end of tRNA(Ala). AARS1 also corrects incorrectly charged tRNA(Ala) through its editing domain. AARS1 Protein, Mouse (sf9, His, solution) is the recombinant mouse-derived AARS1 protein, expressed by Sf9 insect cells , with C-His labeled tag.
研究背景

AARS1 是蛋白质合成中的关键酶，通过两步过程在丙氨酸与 tRNA (Ala) 的附着中发挥着至关重要的作用。最初，丙氨酸被 ATP 激活形成 Ala-AMP，随后转移到 tRNA(Ala) 的受体末端。除了其主要氨酰化功能外，AARS1 还拥有一个编辑域，可以纠正带错误电荷的 tRNA(Ala)，强调了其在维持翻译过程保真度方面的作用。这种双重功能凸显了 AARS1 在确保蛋白质合成机制的准确性和完整性方面的重要性。
生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
种属

Mouse
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

Q8BGQ7 (M1-N968)
基因 ID

234734
蛋白结构
N-term
AARS1 (M1-N968)
Accession # Q8BGQ7
His
C-term
蛋白长度

Full Length
中文名
AARS1 蛋白
同用名
Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase; AlaRS; Sti; Aars
氨基酸序列

MDATLTAREIRERFINFFRRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEARILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFMPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPEMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMNGFEEERRLAQLKSQGKGAGDEDLIMLDIYAIEELRAKGLEATDDSPKYNYQSDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLKVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIVNGMIEAAKPVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPCGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAA

分子量

Approximately 108.43 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
性状

溶液.
组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.
文件资料

COA (209 KB)

产品使用指南 (1213 KB)

AARS1 Protein, Mouse (sf9, His, solution) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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目录号：
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