ABL1 蛋白, Human (GST)

ABL1, Human (GST) is a non-receptor tyrosine-protein kinase. ABL1, Human is implicated in a wide range of cellular processes, including regulation of cell growth and survival, oxidative stress and DNA-damage responses, actin dynamics and cell migration, transmission of information about the cellular environment through integrin signaling.

ABL1 是一种 145 kDa 的蛋白质，包含 N 帽、串联 SH3、SH2 和 SH1 结构域、四个富含脯氨酸的 SH3 结合位点、三个核定位信号、一个核输出信号、一个 DNA 结合结构域和一个肌动蛋白-结合域。ABL1 蛋白涉及广泛的细胞过程，包括细胞生长和存活的调节、氧化应激和 DNA 损伤反应、肌动蛋白动力学和细胞迁移、通过整合素信号传递有关细胞环境的信息。ABL1 蛋白是整合来自各种细胞外和细胞内来源的信号以控制细胞周期和细胞凋亡的关键枢纽^[1][2]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

P00519 (I4-T194)

25  [NCBI]

ICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNT

Approximately 48.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Soverini S, et, al. Chronic myeloid leukemia: the paradigm of targeting oncogenic tyrosine kinase signaling and counteracting resistance for successful cancer therapy. Mol Cancer. 2018 Feb 19;17(1):49.  [Content Brief]

  [2]. Burslem GM, et, al. Targeting BCR-ABL1 in Chronic Myeloid Leukemia by PROTAC-Mediated Targeted Protein Degradation. Cancer Res. 2019 Sep 15;79(18):4744-4753.  [Content Brief]