Acetylcholinesterase/ACHE 蛋白, Mouse (HEK293, His)

Acetylcholinesterase (ACHE) proteins exhibit multiple functions, including acetylcholinesterase activity and protein self-association. It regulates acetylcholine processing, receptor signaling and recycling. Acetylcholinesterase/ACHE Protein, Mouse (HEK293, His) is the recombinant mouse-derived Acetylcholinesterase/ACHE protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Acetylcholinesterase/ACHE Protein, Mouse (HEK293, His) is 583 a.a., with molecular weight of ~80 kDa.

乙酰胆碱酯酶 (ACHE) 蛋白具有多种功能，表现出乙酰胆碱酯酶活性、相同的蛋白结合活性以及蛋白自缔合能力。它参与冷诱导产热的积极调节，使其处于关键过程的上游，例如乙酰胆碱分解代谢过程、乙酰胆碱受体信号传导途径和受体再循环的调节。ACHE 存在于多种细胞成分中，包括基底膜、细胞表面和神经肌肉接头。它的表达跨越多个结构，包括消化系统、泌尿生殖系统、肌肉组织、神经系统和感觉器官。值得注意的是，ACHE 的人类直系同源物与阿尔茨海默病、癫痫和重症肌无力等重要健康状况有关，凸显了该蛋白质在各种生理环境中的重要性。广泛的表达模式进一步强调了其在多种组织中的基本作用，特别是在小脑和胸腺中。

Measured by its ability to cleave Acetylthiocholine. The specific activity is > 250 nmoL/min/μg, as measured under the described conditions.

Mouse

HEK293

C-10*His

NP_033729.1 (E32-L614)

11423  [NCBI]

EGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL

Approximately 80 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.