1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. Aconitase 2/ACO2 蛋白, Mouse (sf9, His-GST)

Aconitase 2/ACO2 蛋白, Mouse (sf9, His-GST)

目录号: HY-P74431
产品使用指南

乌头酸酶2(ACO2)蛋白通过中间体乌头酸催化柠檬酸异构化为异柠檬酸,在细胞代谢中发挥着至关重要的作用。这种酶活性是三羧酸 (TCA) 循环中的关键步骤,而三羧酸循环是能量生产和代谢中间体生成的中心途径。Aconitase 2/ACO2 蛋白, Mouse (sf9, His-GST) 是重组的 乌头酸酶 2/ACO2 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。Aconitase 2/ACO2 蛋白, Mouse (sf9, His-GST) 全长 753 个氨基酸,分子量约为 ~100 kDa。

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描述

Aconitase 2 (ACO2) protein catalyzes the isomerization of citric acid to isocitrate through the intermediate aconitate, playing a crucial role in cellular metabolism. This enzyme activity is a key step in the tricarboxylic acid (TCA) cycle, a central pathway for energy production and generation of metabolic intermediates. Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived Aconitase 2/ACO2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST) is 753 a.a., with molecular weight of ~100 kDa.

研究背景

乌头酸酶 2 (ACO2) 是一种在三羧酸 (TCA) 循环中发挥关键作用的酶,通过中间体顺乌头酸催化柠檬酸异构化为异柠檬酸。这种可逆反应对于碳水化合物的有效代谢和以 NADH 形式产生能量至关重要。ACO2 作为协调 TCA 循环碳流的关键调节剂,有助于生成各种生物合成途径的还原当量和前体。在 ACO2 的促进下,柠檬酸异构化为异柠檬酸,代表了细胞能量产生和代谢稳态的基本步骤。

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q99KI0 (Q28-Q780)

基因 ID

11429  [NCBI]

蛋白结构
N-term
His-GST
ACO2 (Q28-Q780)
Accession # Q99KI0
C-term
同用名
Aconitate hydratase; Aconitase; ACO2
分子量

Approximately 100 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST)
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HY-P74431
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