Acyl-protein thioesterase 2/LYPLA2 蛋白, Human (His)

Acyl-protein thioesterase 2/LYPLA2 Protein, Human (His) a protein palmitoyl thioesterase, is a cytosolic enzyme that catalyze depalmitoylation of membrane-anchored, growth-associated protein-43 (GAP-43).

在哺乳动物中，棕榈酰化由称为棕榈酰酰基转移酶 (PAT)³ 的 23 种酶家族催化，而去棕榈酰化由四种硫酯酶催化。其中两种硫酯酶，酰基蛋白硫酯酶-1 (APT1) 和 APT2，主要位于细胞质中，另外两种，棕榈酰蛋白硫酯酶-1 (PPT1) 和 PPT2，是溶酶体酶。据报道，酰基蛋白硫酯酶 2 (APT2) 可以使 GAP-43（生长相关蛋白 43）去棕榈酰^[1]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

C-6*His

O95372 (M1-V231)

11313  [NCBI]

MCGNTMSVPLLTDAATVSGAERETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGLSPDAPEDEAGIKKAAENIKALIEHEMKNGIPANRIVLGGFSQGGALSLYTALTCPHPLAGIVALSCWLPLHRAFPQAANGSAKDLAILQCHGELDPMVPVRFGALTAEKLRSVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLLPPV

Approximately 28.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 1 mM DTT, 0.1 M NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Eryan Kong, et al. Dynamic palmitoylation links cytosol-membrane shuttling of acyl-protein thioesterase-1 and acyl-protein thioesterase-2 with that of proto-oncogene H-ras product and growth-associated protein-43. J Biol Chem. 2013 Mar 29;288(13):9112-25.  [Content Brief]