ADAM15 蛋白, Mouse (HEK293, His)

ADAM15 is an active metalloprotease that plays a crucial role in physiological processes and has gelatin-solubilizing and collagen-solubilizing activities. Notably, it affects wound healing, mediates intraepithelial cell/T cell interactions, and inhibits β-1 integrin-mediated cell adhesion and migration in airway smooth muscle cells. ADAM15 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ADAM15 protein, expressed by HEK293 , with C-His labeled tag. The total length of ADAM15 Protein, Mouse (HEK293, His) is 680 a.a., with molecular weight of 75-110 kDa.

ADAM15 是一种活性金属蛋白酶，具有分解明胶和分解胶原的活性，在多种生理过程中发挥着至关重要的作用。它在伤口愈合中的作用值得注意，并且介导异型上皮内细胞/T 细胞相互作用和同型 T 细胞聚集。此外，ADAM15 还可抑制 β-1 整合素介导的气道平滑肌细胞粘附和迁移，通过 ERK1/2 失活潜在影响 α-v/β-1 整合素细胞表面表达，从而抑制细胞在纤连蛋白上或向纤连蛋白运动。值得注意的是，ADAM15 响应生长因子剥夺而裂解 E-钙粘蛋白，并有助于肾小球细胞迁移，表明其参与各种细胞事件。此外，它在病理性新生血管形成中的作用和对软骨重塑的潜在贡献强调了它的多方面功能。ADAM15 可能在精子附睾成熟和顶体反应过程中经历蛋白水解过程，可能通过与卵膜受体（特别是整合素）相互作用在精卵结合中发挥作用。

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 4.53 μg/mL, corresponding to a specific activity is 2.21×10² units/mg.

Mouse

HEK293

C-10*His

O88839 (R18-T697)

11490  [NCBI]

RPSPPLPNIGGTEEEQQASPERTLSGSMESRVVQDSPPMSLADVLQTGLPEALRISLELDSESHVLELLQNRDLIPGRPTLVWYQPDGTRMVSEGYSLENCCYRGRVQGHPSSWVSLCACSGIRGLIVLSPERGYTLELGPGDLQRPVISRIQDHLLLGHTCAPSWHASVPTRAGPDLLLEQHHAHRLKRDVVTETKIVELVIVADNSEVRKYPDFQQLLNRTLEAALLLDTFFQPLNVRVALVGLEAWTQHNLIEMSSNPAVLLDNFLRWRRTDLLPRLPHDSAQLVTVTSFSGPMVGMAIQNSICSPDFSGGVNMDHSTSILGVASSIAHELGHSLGLDHDSPGHSCPCPGPAPAKSCIMEASTDFLPGLNFSNCSRQALEKALLEGMGSCLFERQPSLAPMSSLCGNMFVDPGEQCDCGFPDECTDPCCDHFTCQLRPGAQCASDGPCCQNCKLHPAGWLCRPPTDDCDLPEFCPGDSSQCPSDIRLGDGEPCASGEAVCMHGRCASYARQCQSLWGPGAQPAAPLCLQTANTRGNAFGSCGRSPGGSYMPCAPRDVMCGQLQCQWGRSQPLLGSVQDRLSEVLEANGTQLNCSWVDLDLGNDVAQPLLALPGTACGPGLVCIGHRCQPVDLLGAQECRRKCHGHGVCDSSGHCRCEEGWAPPDCMTQLKATSSLTT

Approximately 75-110 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.