ADAMDEC1 蛋白, Human (HEK293, His)

ADAMDEC1 Protein, Human (HEK293, His) is human recombinant ADAM-like protein decysin-1 (ADAMDEC1) with a 6His tag at the C-terminus. ADAMDEC1 Protein, Human (HEK293, His) is produced by mammalian expression system in human cells and the target gene encoding Ile31-Glu470 is expressed.

ADAM 样 Decysin-1 (ADAMDEC1) 是 ADAM 家族的成员，是一种分泌型金属蛋白酶。ADAMDEC1 包含信号肽、前结构域、催化结构域和不完整的解整合素结构域。ADAMDEC1 包含具有蛋白水解活性的 ADAM 共有序列，并在体外表现出 α2-巨球蛋白 (α2M) 的金属蛋白酶活性。ADAMDEC1 的表达在正常和 RasV12 转化的上皮细胞的混合培养物中上调，这有利于转化细胞的顶端挤出^[1]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

O15204 (K206-E470)

27299  [NCBI]

KSPEKEDFLRAQKYIDLYLVLDNAFYKNYNENLTLIRSFVFDVMNLLNVIYNTIDVQVALVGMEIWSDGDKIKVVPSASTTFDNFLRWHSSNLGKKIHDHAQLLSGISFNNRRVGLAASNSLCSPSSVAVIEAKKKNNVALVGVMSHELGHVLGMPDVPFNTKCPSGSCVMNQYLSSKFPKDFSTSCRAHFERYLLSQKPKCLLQAPIPTNIMTTPVCGNHLLEVGEDCDCGSPKECTNLCCEALTCKLKPGTDCGGDAPNHTTE

Approximately 38.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris, 150 mM NaCl, 1 mM ZnCl₂, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Yako Y, et al. ADAM-like Decysin-1 (ADAMDEC1) is a positive regulator of Epithelial Defense Against Cancer (EDAC) that promotes apical extrusion of RasV12-transformed cells. Sci Rep. 2018 Jun 25;8(1):9639.  [Content Brief]