1. 重组蛋白
  2. Enzymes & Regulators
  3. ADAMs/ADAMTSs
  4. ADAMTSL-1/Punctin-1
  5. ADAMTSL1/Punctin 蛋白, Human (sf9, His)

ADAMTSL1/Punctin 蛋白, Human (sf9, His)

目录号: HY-P76135
COA 产品使用指南

ADAMTSL1/Punctin 蛋白是 ADAMTS 家族的成员,编码一种独特的分泌蛋白,缺乏金属蛋白酶和解整合素样结构域,但保留其他 ADAMTS 结构域,包括血小板反应蛋白 1 型基序。选择性剪接可能涉及关键的细胞外基质功能,产生多个转录变体,每个变体编码不同的蛋白质。ADAMTSL1/Punctin 蛋白, Human (sf9, His) 是重组的 ADAMTSL1/Punctin 蛋白,由 Sf9 昆虫细胞表达,带有 C-His 标签。ADAMTSL1/Punctin 蛋白, Human (sf9, His) 全长 439 个氨基酸,分子量约为 ~48 KDa。

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100 μg ¥6500 In-stock
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描述

ADAMTSL1/Punctin Protein, a member of the ADAMTS family, encodes a unique secreted protein lacking metalloproteinase and disintegrin-like domains but retaining other ADAMTS domains, including the thrombospondin type 1 motif. Likely involved in crucial extracellular matrix functions, alternative splicing generates multiple transcript variants, each encoding distinct proteins. ADAMTSL1/Punctin Protein, Human (sf9, His) is the recombinant human-derived ADAMTSL1/Punctin protein, expressed by Sf9 insect cells, with C-His labeled tag. The total length of ADAMTSL1/Punctin Protein, Human (sf9, His) is 439 a.a., with molecular weight of ~48 KDa.

研究背景

ADAMTSL1 是 ADAMTS(一种具有血小板反应蛋白基序的解整合素和金属蛋白酶)家族的成员,编码一种分泌蛋白。与典型的 ADAMTS 家族成员相比,该蛋白缺乏金属蛋白酶和解整合素样结构域,但保留了其他 ADAMTS 结构域,包括血小板反应蛋白 1 型基序。该蛋白质可能参与细胞外基质内的关键功能。选择性剪接产生多个转录变体,每个变体编码不同的蛋白质。

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q8N6G6-2 (M1-H439)

基因 ID
蛋白结构
N-term
ADAMTSL1 (M1-H439)
Accession # Q8N6G6-2
His
C-term
同用名
ADAMTS-like protein 1; ADAMTSL-1; Punctin-1; ADAMTSR1; C9orf94
分子量

Approximately 48 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

ADAMTSL1/Punctin Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ADAMTSL1/Punctin Protein, Human (sf9, His)
目录号:
HY-P76135
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