Adenylate Kinase 1/AK1 蛋白, Human (His)

Adenylate Kinase 1/AK1 Protein is an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. AK1 is highly expressed in skeletal muscle, brain and erythrocytes. It facilitates cellular energy dynamics by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. In addition, AK1 displays nucleoside diphosphate kinase activity. Adenylate Kinase 1/AK1 Protein, Human (His) is the recombinant human-derived Adenylate Kinase 1/AK1 protein, expressed by E. coli , with N-His labeled tag. The total length of Adenylate Kinase 1/AK1 Protein, Human (His) is 194 a.a., with molecular weight of ~23 kDa.

腺苷酸激酶 1/AK1 是一种腺苷酸激酶，参与不同细胞内区室中能量代谢和细胞腺嘌呤核苷酸比例的稳态。AK1 在骨骼肌、大脑和红细胞中高度表达。它通过催化 ATP 和 AMP 之间末端磷酸基团的可逆转移来促进细胞能量动态。除了其主要作用外，AK1 还表现出核苷二磷酸激酶活性，利用 ATP 或 GTP 作为磷酸盐供体，从相应的二磷酸底物中生成 ATP、CTP、GTP、UTP、dATP、dCTP、dGTP 和 dTTP。此外，AK1 对从二磷酸硫胺素 (ThDP) 和 ADP 合成三磷酸硫胺素 (ThTP) 具有低速率催化作用。AK1 的多功能酶活性强调了其在维持腺苷酸核苷酸平衡方面的重要性及其在更广泛的细胞过程中的潜在作用，超越了传统的核苷酸代谢^[1][2]。

Specific activity is 120.42 pmol/min/μg. One unit will convert 1 pmoles of AMP and ATP to 2 pmoles of ADP per minute at 37°C.

Human

E. coli

N-6*His

AAH01116 (M1-K194)

203  [NCBI]

MEEKLKKTNIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRSEVSSGSARGKKLSEIMEKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDSVFSQVCTHLDALK

Approximately 23 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, 10% glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.