2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. ADH II 蛋白, Zymomonas mobilis subsp. mobilis

ADH II 蛋白, Zymomonas mobilis subsp. mobilis

目录号: HY-P701933
产品使用指南

ADH II 蛋白是一种铁依赖性乙醇脱氢酶，对于将丙酮酸转化为乙醇至关重要。这种酶活性对于涉及乙醇形成的途径至关重要，有助于酒精代谢的生理过程。它的铁依赖性强调了复杂的生化机制，强调了 ADH II 在相关生物背景下从丙酮酸生产乙醇的重要性。ADH II 蛋白, Zymomonas mobilis subsp. mobilis 是重组的 ADH II 蛋白，由 E. coli 表达，不带标签。ADH II 蛋白, Zymomonas mobilis subsp. mobilis 全长 382 个氨基酸。

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描述

ADH II Protein, an iron-dependent alcohol dehydrogenase, is pivotal in converting pyruvate to ethanol. This enzymatic activity is essential for pathways involving ethanol formation, contributing to physiological processes in alcohol metabolism. Its iron dependency underscores intricate biochemical mechanisms, emphasizing ADH II's significance in producing ethanol from pyruvate within relevant biological contexts. ADH II Protein, Zymomonas mobilis subsp. mobilis is the recombinant ADH II protein, expressed by E. coli , with tag free. The total length of ADH II Protein, Zymomonas mobilis subsp. mobilis is 382 a.a., .
研究背景

ADH II 是一种铁依赖性乙醇脱氢酶，在丙酮酸转化为乙醇的过程中发挥着关键作用。这种酶活性对于涉及乙醇形成的代谢途径至关重要，有助于与酒精代谢相关的生理过程。铁依赖性强调了控制这种转化的复杂生化机制，突出了 ADH II 在促进相关生物背景下从丙酮酸生产乙醇方面的重要性。
种属

Others
表达系统

E. coli
标签

Tag Free
蛋白编号

P0DJA2 (A2-F383)
基因 ID

79905068
蛋白结构
N-term
ADH II (A2-F383)
Accession # P0DJA2
C-term
同用名
adhB; Alcohol dehydrogenase 2; Alcohol dehydrogenase II; ADH II
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

ADH II Protein, Zymomonas mobilis subsp. mobilis 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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