1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. ADH4 蛋白, Saccharomyces cerevisiae (His, Strep)

ADH4 蛋白, Saccharomyces cerevisiae (His, Strep)

目录号: HY-P701929
产品使用指南

ADH4 蛋白是一种特定的乙醇脱氢酶,主要充当线粒体甲醛脱氢酶,具有独特的乙醇偏好。它不影响乙醇生产,并且对具有四个或更多碳原子的伯醇表现出降低的活性。ADH4 蛋白, Saccharomyces cerevisiae (His, Strep) 是重组的 ADH4 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。ADH4 蛋白, Saccharomyces cerevisiae (His, Strep) 全长 381 个氨基酸。

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描述

The ADH4 protein is a specific alcohol dehydrogenase that primarily functions as a mitochondrial formaldehyde dehydrogenase with a unique ethanol preference. It does not affect ethanol production and exhibits reduced activity on primary alcohols with four or more carbon atoms. ADH4 Protein, Saccharomyces cerevisiae (His, Strep) is the recombinant ADH4 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of ADH4 Protein, Saccharomyces cerevisiae (His, Strep) is 381 a.a., .

研究背景

ADH4 蛋白作为一种特定的乙醇脱氢酶,对乙醇具有明显的偏好,主要作为线粒体甲醛脱氢酶,而不影响乙醇的产生。其酶活性对于含有四个或更多碳原子的伯醇显着降低,而脂肪醇、仲醇和甘油的异构体则不表现出反应性。虽然 ADH4 在酵母代谢中的确切作用尚不清楚,但已确定 ADH4 在葡萄糖生长过程中在乙醇生产中不发挥作用,也不参与乙醇氧化为乙醛。对底物特异性和功能特征的描述提供了对 ADH4 在细胞过程中的特定作用的深入了解,揭示了其对酒精代谢和线粒体甲醛氧化的独特贡献。

种属

Others

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

P10127 (S2-Y382)

基因 ID

852636

蛋白结构
N-term
Strep-6*His
ADH4 (S2-Y382)
Accession # P10127
C-term
同用名
ADH4; Alcohol dehydrogenase 4; Alcohol dehydrogenase IV; ADHIV
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ADH4 Protein, Saccharomyces cerevisiae (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ADH4 Protein, Saccharomyces cerevisiae (His, Strep)
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