1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Peptide Hormone & Neuropeptides
  4. Adiponectin
  5. Adiponectin/Acrp30 蛋白, Human (HEK293, His)

Adiponectin/Acrp30 蛋白, Human (HEK293, His)

目录号: HY-P70504
COA 产品使用指南

Adiponectin/Acrp30 蛋白, Human (HEK293, His)可提高细胞对胰岛素的敏感性,也可作为治疗糖尿病性肌腱病的潜在蛋白。

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥300 In-stock
10 μg ¥680 In-stock
50 μg ¥1700 In-stock
100 μg ¥2700 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Adiponectin/Acrp30 Protein, Human (HEK293, His) could increase cell sensitivity to insulin and can be used as a potential protein for treating diabetic tendinopathy[1].

研究背景

重组人脂联素可用于受损动脉并减轻血管炎症反应。据报道,生理浓度的重组人脂联素可抑制肿瘤坏死因子-α (TNF-α) 诱导的内皮粘附分子表达、巨噬细胞向泡沫细胞的转化以及巨噬细胞中 TNF-α 的表达[1]。重组人脂联素可作为治疗糖尿病肌腱病的潜在蛋白质,在体外促进肌腱细胞祖细胞增殖和肌腱分化[2]

生物活性

Measured by its ability to inhibit glucose production by rat hepatocytes. The ED50 for this effect is 5.936 µg/mL, corresponding to a specific activity is 168.46 units/mg.

  • Measured by its ability to inhibit glucose production by rat hepatocytes. The ED50 for this effect is 5.936 µg/mL, corresponding to a specific activity is 168.46 units/mg.
种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q15848 (E19-N244)

基因 ID
蛋白结构
N-term
Acrp30 (E19-N244)
Accession # Q15848
His
C-term
中文名
重组人 Adipolean/gAcrp30 (HEK293, His)
同用名
Adiponectin; 30 kDa Adipocyte Complement-Related Protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte; C1q and Collagen Domain-Containing Protein; Adipose Most Abundant Gene Transcript 1 Protein; apM-1; Gelatin-Binding Protein; ADIPOQ; ACDC; ACRP3
氨基酸序列

ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN

分子量

32-40 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM Tris-HCL, 150 mM NaCL, 10% Glycerol, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Adiponectin/Acrp30 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Adiponectin/Acrp30 Protein, Human (HEK293, His)
目录号:
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