Agrin 蛋白, Human (1073a.a, CHO, His)

Agrin is a heparan sulfate basal glycoprotein that is critical for the formation and maintenance of the neuromuscular junction (NMJ). It forms the AGRN-LRP4 receptor complex, activates MUSK and regulates gene transcription and AChR clusters in the postsynaptic membrane to form the NMJ. Agrin Protein, Human (1073a.a, CHO, His) is the recombinant human-derived Agrin protein, expressed by CHO , with C-6*His labeled tag. The total length of Agrin Protein, Human (1073a.a, CHO, His) is 1073 a.a., with molecular weight of approximately 155.89 kDa.

Agrin 是一种硫酸乙酰肝素基底层糖蛋白，对于神经肌肉接头 (NMJ) 的形成和维持至关重要，协调突触后分化中的关键事件。作为 AGRN-LRP4 受体复合物的组成部分，它诱导 MUSK 的磷酸化和激活，通过调节突触后膜中的基因转录和 AChR 聚类等过程导致 NMJ 形成，其中钙离子起着关键作用。Agrin 的神经元功能受到选择性剪接、聚糖结合和蛋白水解加工的复杂调节。在神经元中，它调节钙离子稳态，诱导细胞质钙离子增加。Agrin 还通过抑制 Na+/K+-ATP 酶的 α(3) 亚型，在 CNS 突触处引起去极化，从而在中枢神经系统 (CNS) 中发挥不同作用。分泌的异构体通过结合层粘连蛋白充当运动神经元和基底层之间的桥梁，而跨膜形式主要存在于脑神经元中，通过糖胺聚糖链和 Rho 家族 GTP 酶的作用诱导成熟海马神经元的树突状丝状伪足和突触形成。

Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. The ED₅₀ for this effect is 195.7 ng/ml, corresponding to a specific activity is 5.11×10^3 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. The ED₅₀ for this effect is 195.7ng/mL, corresponding to a specific activity is 5.11×10³ units/mg.

Human

CHO

C-6*His

O00468 (T30-R1102)

375790  [NCBI]

TCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVER

approximately 155.89 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.