AIFM2 蛋白, Human (Strep)

AIFM2 Protein, Human (Strep II) is the recombinant human-derived AIFM2, expressed by E. coli, with Strep II labeled tag.

AIFM2 是一种依赖 NAD(P)H 的氧化还原酶，作为铁死亡（ferroptosis）的关键抑制剂。在质膜上，它能催化辅酶 Q/泛醌-10 还原为泛醇-10，这是一种亲脂性自由基捕获抗氧化剂，可防止脂质氧化损伤从而抑制铁死亡。其作用与 GPX4 平行，共同抑制磷脂过氧化和铁死亡，且这一抗铁死亡功能不依赖于细胞谷胱甘肽水平。此外，AIFM2 还通过介导华法林抗性的维生素 K 还原（在经典维生素 K 循环中）发挥强效自由基捕获抗氧化作用：催化 NAD(P)H 依赖的维生素 K（叶绿醌、甲基萘醌-4 和甲萘醌）还原为氢醌形式，后者作为强效自由基捕获抗氧化剂抑制磷脂过氧化和铁死亡。AIFM2 可能参与线粒体应激信号传导：在氧化应激下，它与脂质过氧化终产物 4-羟基-2-壬烯醛（HNE）结合形成无氧化还原酶活性的脂质加合物，随后从线粒体转位至细胞核引发 DNA 损伤和细胞死亡。该酶还能以非序列特异性方式结合 DNA。

Human

E. coli

C-StrepⅡ

Q9BRQ8-1 (G2-P373)

84883

Full Length of Mature Protein

Approximately 40.6 kDa

Greater than 85% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.