1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. TNF Superfamily
  4. TNF Superfamily Ligands
  5. GITRL/AITRL
  6. AITRL/TNFSF18 Trimer 蛋白, Human (Biotinylated, HEK293, His-Flag)

AITRL/TNFSF18 Trimer 蛋白, Human (Biotinylated, HEK293, His-Flag)

目录号: HY-P78133
COA 产品使用指南

AITRL,一种 II 型跨膜蛋白,是糖皮质激素诱导的 TNFR 相关蛋白 (GITR) 的配体。当 AITRL 与 GITR 结合时,GITR 可以产生调节 T 细胞增殖和效应子功能的共刺激信号。GITR/GITRL 相互作用在肿瘤、炎症以及自身免疫性疾病的发病中发挥着重要作用。此外,AITRL 还在内皮细胞 (ECs) 的激活中发挥作用,并促进小鼠和人类细胞的粘附,从而增加 STAT-1 磷酸化和粘附分子 (如 VCAM-1 和 ICAM-1) 的表达。AITRL/TNFSF18 蛋白, Human (Biotinylated, HEK293, His-Flag) 是一种重组生物素化的人 AITRL (Q72-S199),其 N 端带有 His 和 Flag 标签,在 HEK293 中表达。

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20 μg ¥2600 In-stock
50 μg ¥5700 In-stock
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250 μg   询价  

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描述

AITRL, a type II transmembrane protein, is a ligand for glucocorticoid-induced TNFR-related protein (GITR). When AITRL binds to GITR, GITR can produce costimulatory signals that regulate T-cell proliferation and effector functions. GITR/AITRL interaction plays a role in the pathogenesis of tumor, inflammation, as well as autoimmune diseases[1]. Besides, AITRL plays a role in endothelial cells (EC)-activation and increases STAT-1 phosphorylation and the expression of adhesion molecules (VCAM-1, ICAM-1)[2]. AITRL/TNFSF18 Protein, Human (Biotinylated, HEK293, His-Flag) is a recombinant biotinylated human AITRL (Q72-S199) with N-terminal His and Flag tag, which is expressed in HEK293.

研究背景

GITRL (AITRL) 是一种 II 型跨膜蛋白,是糖皮质激素诱导的 TNFR 相关蛋白 (GITR) 的配体。GITR 是 TNFR 超家族的成员,在 T 细胞、自然杀伤细胞和一些骨髓细胞中表达。而GITRL主要表达于抗原呈递细胞(B细胞、树突状细胞)、巨噬细胞和内皮细胞(EC)[1]
当GITRL与GITR结合时,GITR可产生共刺激信号调节 T 细胞增殖和效应功能。这种相互作用刺激 CD4+ Teff 和 Treg 细胞的增殖和细胞因子产生,并驱动 CD8+ T 细胞[3]的抗肿瘤活性。此外,GITRL 在 EC 激活中发挥作用,并促进小鼠和人类的粘附,从而增加 STAT-1 磷酸化并增强 VCAM-1 和 ICAM-1 等粘附分子的表达[2]
人类 GITRL 与小鼠的共同氨基酸一致性< 55%。人 GITRL 由胞质结构域 (M1-W27)、螺旋结构域 (L28-F48) 和胞外结构域 (L49-S177) 组成。人GITRL是一个三聚体,但也可以是单体或组装成其他多聚体结构[4]
GITR/GITRL相互作用在肿瘤、炎症等的发病机制中发挥着重要作用作为自身免疫性疾病[1]

体外研究
(In Vitro)

AITRL (human, 48 h) reduces the suppressive capacity of MDSCs on CD4+ T cells (MDSCs induced from healthy donors)[5].

生物活性

1.Immobilized Human GITR His at 1 μg/mL (100 μL/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer His with the EC50 of 0.2 μg/mL determined by ELISA.
2.Immobilized Human GITR His at 5 μg/mL (100 μL/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer His with the EC50 of 1.69 μg/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

N-His;N-Flag

蛋白编号

Q9UNG2 (Q50-S177)

基因 ID
中文名
重组人 AITRL/TNFSF18 三聚体蛋白 (Biotinylated, HEK293, His-Flag)
同用名
TNFSF18; AITRL; TL6; GITRL; GITR Ligand
氨基酸序列

QLETAKEPCMAKFGPLPSKWQMASSEPPCVNKVSDWKLEILQNGLYLIYGQVAPNANYNDVAPFEVRLYKNKDMIQTLTNKSKIQNVGGTYELHVGDTIDLIFNSEHQVLKNNTYWGIILLANPQFIS

分子量

50-70 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

AITRL/TNFSF18 Trimer Protein, Human (Biotinylated, HEK293, His-Flag) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
AITRL/TNFSF18 Trimer Protein, Human (Biotinylated, HEK293, His-Flag)
目录号:
HY-P78133
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