1. 重组蛋白
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. AKT Serine/Threonine Kinase 3 (AKT3)
  5. AKT3 蛋白, Mouse (sf9)

AKT3 蛋白, Mouse (sf9)

目录号: HY-P72817
产品使用指南

AKT3 蛋白是 AKT 激酶家族的一部分,调节多种细胞过程,包括代谢、增殖和存活。其研究最少的亚型 AKT3 对大脑发育至关重要,对恶性神经胶质瘤细胞的活力也至关重要。 AKT3 还在协调线粒体生物合成与能量需求以及通过 IL13 调节 MMP13 表达方面发挥着作用。 RNA 干扰诱导的下调导致磷酸化 BAD 减少并触发 caspase 依赖性细胞凋亡。AKT3 蛋白, Mouse (sf9) 是重组的 AKT3 蛋白,由 Sf9 insect cells 表达,不带标签。AKT3 蛋白, Mouse (sf9) 全长 374 个氨基酸,分子量约为 ~46 kDa。

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描述

AKT3 Protein, part of the AKT kinase family, regulates diverse cellular processes, including metabolism, proliferation, and survival. Its least studied isoform, AKT3, is vital for brain development and essential for malignant glioma cell viability. AKT3 also plays a role in coordinating mitochondrial biogenesis with energy demands and modulating MMP13 expression via IL13. RNA interference-induced down-regulation leads to decreased phosphorylated BAD and triggers caspase-dependent apoptosis. AKT3 Protein, Mouse (sf9) is the recombinant mouse-derived AKT3 protein, expressed by Sf9 insect cells , with tag free. The total length of AKT3 Protein, Mouse (sf9) is 374 a.a., with molecular weight of ~46 kDa.

研究背景

AKT3AKT 激酶家族的成员,与 AKT1AKT2 一样,是一种丝氨酸/苏氨酸蛋白激酶,可协调多种重要的细胞过程,包括代谢、增殖、细胞存活、生长和血管生成。它的影响是通过众多下游底物的磷酸化来发挥的,已鉴定出 100 多种潜在候选物,尽管大多数都缺乏报道的亚型特异性。尽管 AKT3 是研究最少的同种型,但它已成为大脑发育的关键参与者,并被证明对于恶性神经胶质瘤细胞的生存能力不可或缺。值得注意的是,它可能作为通过 IL13 上调和下调 MMP13 的中心介质。此外,AKT3 在协调线粒体生物发生与生长因子引发的细胞能量需求增加方面发挥着至关重要的作用。通过 RNA 干扰沉默 AKT3 表达可下调 BAD 的磷酸化形式,最终诱导 caspase 依赖性细胞凋亡,强调其多方面且重要的调节功能。

种属

Mouse

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q9WUA6-1 (A106-E479)

基因 ID
蛋白结构
N-term
AKT3 (A106-E479)
Accession # Q9WUA6-1
C-term
同用名
RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; PKB gamma; Akt3
氨基酸序列

ADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFDYLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDEVAHTLTESRVLKNTRHPFLTSLKYSFQTKDRLCFVMEYVNGGELFFHLSRERVFSEDRTRFYGAEIVSALDYLHSGKIVYRDLKLENLMLDKDGHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKYDDDGMDGMDNERRPHFPQFSYSASGRE

分子量

Approximately 46 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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