1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. ALDH4A1 蛋白, Human (sf9)

ALDH4A1 蛋白, Human (sf9)

目录号: HY-P76138
COA 产品使用指南

ALDH4A1 蛋白是一种关键酶,可将 delta-1-吡咯啉-5-羧酸盐 (P5C) 不可逆地转化为谷氨酸盐,这是连接尿素和三羧酸循环的关键步骤。ALDH4A1 更喜欢谷氨酸 γ-半醛,但也接受琥珀酸、戊二酸和己二酸半醛等底物。ALDH4A1 蛋白, Human (sf9) 是重组的 ALDH4A1 蛋白,由 Sf9 insect cells 表达,不带标签。ALDH4A1 蛋白, Human (sf9) 全长 539 个氨基酸,分子量约为 ~54 KDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The ALDH4A1 protein is a key enzyme that irreversibly converts delta-1-pyrroline-5-carboxylate (P5C) to glutamate, a key step linking the urea and tricarboxylic acid cycles. ALDH4A1 prefers glutamate γ-semialdehyde but also accepts substrates such as succinate, glutaric acid, and adipic acid semialdehyde. ALDH4A1 Protein, Human (sf9) is the recombinant human-derived ALDH4A1 protein, expressed by Sf9 insect cells , with tag free. The total length of ALDH4A1 Protein, Human (sf9) is 539 a.a., with molecular weight of ~54 KDa.

研究背景

ALDH4A1 是一种关键酶,可催化源自脯氨酸或鸟氨酸的 delta-1-吡咯啉-5-羧酸盐 (P5C) 不可逆地转化为谷氨酸。这种酶促转化是连接尿素和三羧酸循环的复杂途径中的关键步骤。ALDH4A1 的首选底物是谷氨酸 γ-半醛,同时它也接受其他底物,例如琥珀酸、戊二酸和己二酸半醛。ALDH4A1 的酶活性在维持脯氨酸、鸟氨酸和谷氨酸之间的代谢平衡中发挥着核心作用,有助于在相互关联的细胞途径中有效利用这些氨基酸。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

P30038 (K25-Q563)

基因 ID
蛋白结构
N-term
ALDH4A1 (K25-Q563)
Accession # P30038
C-term
同用名
Delta-1-pyrroline-5-carboxylate dehydrogenase; P5C dehydrogenase; ALDH4; P5CDH
分子量

Approximately 54 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

ALDH4A1 Protein, Human (sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ALDH4A1 Protein, Human (sf9)
目录号:
HY-P76138
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