1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. ALG13 蛋白, Human (His)

ALG13 蛋白, Human (His)

目录号: HY-P701457
产品使用指南

ALG13 蛋白被认为是一种具有糖基转移酶和去泛素酶活性的多功能酶,在蛋白质 N-糖基化的多醇连接寡糖途径的第二步中发挥作用。这意味着 ALG13 参与重要的细胞过程、桥接糖基化和去泛素化活性,并强调其在调节蛋白质修饰和细胞途径中的潜在重要性。ALG13 蛋白, Human (His) 是重组的 ALG13 蛋白,由 E. coli 表达,带有 N-6*His 标签。ALG13 蛋白, Human (His) 全长 152 个氨基酸。

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描述

ALG13 protein is suggested to be a multifunctional enzyme with glycosyltransferase and deubiquitinase activities, playing a role in the second step of the dolichol-linked oligosaccharide pathway for protein N-glycosylation. This implies ALG13's involvement in vital cellular processes, bridging glycosylation and deubiquitination activities and highlighting its potential significance in regulating protein modifications and cellular pathways. ALG13 Protein, Human (His) is the recombinant human-derived ALG13 protein, expressed by E. coli , with N-6*His labeled tag. The total length of ALG13 Protein, Human (His) is 152 a.a., .

研究背景

ALG13 蛋白被认为是一种潜在的多功能酶,具有糖基转移酶和去泛素酶活性。它被认为在多醇连接寡糖途径的第二步中发挥作用,特别是在蛋白质 N-糖基化中。ALG13 的多方面性质意味着它参与重要的细胞过程、桥接糖基化和去泛素化活性,从而突出了其在调节蛋白质修饰和细胞途径中的潜在重要性。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9NP73 (S221-D372)

基因 ID

79868

蛋白结构
N-term
6*His
ALG13 (S221-D372)
Accession # Q9NP73
C-term
同用名
ALG13; Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13; Asparagine-linked glycosylation 13 homolog; Glycosyltransferase 28 domain-containing protein 1; UDP-N-acetylglucosamine transferase subunit ALG13 homolog
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ALG13 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ALG13 Protein, Human (His)
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