Alpha-2-Antiplasmin/Serpin F2 蛋白, Human (HEK293, His)

Alpha-2-Antiplasmin/Serpin F2 Protein, a serine protease inhibitor, primarily targets plasmin and trypsin, also inactivating matriptase-3/TMPRSS7 and chymotrypsin. It can form a heterodimer with TMPRSS7, enhancing its protease inhibitory activity. Alpha-2-Antiplasmin/Serpin F2 Protein, Human (HEK293, His) is the recombinant human-derived Alpha-2-Antiplasmin/Serpin F2 protein, expressed by HEK293 , with C-His labeled tag. The total length of Alpha-2-Antiplasmin/Serpin F2 Protein, Human (HEK293, His) is 491 a.a., with molecular weight of ~70 kDa.

Alpha-2-Antiplasmin/Serpin F2 蛋白是一种丝氨酸蛋白酶抑制剂，主要针对纤溶酶和胰蛋白酶，但它也会灭活 matriptase-3/TMPRSS7 和糜蛋白酶。此外，它能够与TMPRSS7形成蛋白酶抑制异二聚体，从而增强其抑制活性。

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate,Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec) . The IC₅₀ value is < 0.5 nM .

Human

HEK293

C-His

P08697 (M1-K491)

5345  [NCBI]

MALLWGLLVLSWSCLQGPCSVFSPVSAMEPLGRQLTSGPNQEQVSPLTLLKLGNQEPGGQTALKSPPGVCSRDPTPEQTHRLARAMMAFTADLFSLVAQTSTCPNLILSPLSVALALSHLALGAQNHTLQRLQQVLHAGSGPCLPHLLSRLCQDLGPGAFRLAARMYLQKGFPIKEDFLEQSEQLFGAKPVSLTGKQEDDLANINQWVKEATEGKIQEFLSGLPEDTVLLLLNAIHFQGFWRNKFDPSLTQRDSFHLDEQFTVPVEMMQARTYPLRWFLLEQPEIQVAHFPFKNNMSFVVLVPTHFEWNVSQVLANLSWDTLHPPLVWERPTKVRLPKLYLKHQMDLVATLSQLGLQELFQAPDLRGISEQSLVVSGVQHQSTLELSEVGVEAAAATSIAMSRMSLSSFSVNRPFLFFIFEDTTGLPLFVGSVRNPNPSAPRELKEQQDSPGNKDFLQSLKGFPRGDKLFGPDLKLVPPMEEDYPQFGSPK

Approximately 70 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 25 mM Tris, 150 mM NaCl, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.