Alpha-2-macroglobulin 蛋白, Human (sf9, His)

Alpha-2-macroglobulin Protein, a protease inhibitor and cytokine transporter, belongs to the MEROPS proteinase inhibitor family I39, clan IL. A2M acts on all classes of endoproteases and could potentially be used to slow or halt PTOA by neutralizing catabolic cascades in affected cartilage. A2M can enter into cells and bind to numerous cytokines. It inhibits inflammatory mediators of chondrocytes by blocking IL-1尾/NF-魏B pathway. Alpha-2-macroglobulin Protein, Human (sf9, His) is the recombinant human-derived Alpha-2-macroglobulin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Alpha-2-macroglobulin Protein, Human (sf9, His) is 1451 a.a., with molecular weight of 160-170 kDa.

Alpha-2-巨球蛋白 (A2M) 是一种蛋白酶抑制剂和细胞因子转运蛋白，属于 MEROPS 蛋白酶抑制剂家族 I39，IL 族。它也是血液中发现的大血浆蛋白。主要由肝脏产生，也由巨噬细胞、成纤维细胞和肾上腺皮质细胞局部合成。该抑制剂作用于所有类别的内切蛋白酶，并可能通过中和受影响软骨中的分解代谢级联来减缓或停止 PTOA。A2M可以进入细胞并与多种细胞因子结合，包括转化生长因子-β1 (TGF-β1)、TGF-β2、神经生长因子-β、血小板源性生长因子-BB、肿瘤坏死因子-α (TNF- α) 和碱性成纤维细胞生长因子。A2M 通过阻断 IL-1β/NF-κB 通路来抑制软骨细胞的炎症介质。A2M 作为骨质疏松症中的一种新型疾病调节蛋白，其在骨髓中的下调会促进 SSPC 功能障碍和骨骼稳态失衡^[1][2][3]。

Measured by its ability to trap trypsin. The trapped trypsin is no longer able to interact with protein substrates or inhibitors, but still able to cleave small peptide substrates or inhibitors.The IC₅₀ value is <5 nM.

Human

Sf9 insect cells

C-His

NP_000005.2 (S24-A1474)

2  [NCBI]

SVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQDDEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA

160-170 kDa

Greater than 85% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl, 20% glycerol, pH 7.4

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.