1. 重组蛋白
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. AMIGO2 蛋白, Mouse (HEK293, His)

AMIGO2 蛋白, Mouse (HEK293, His)

目录号: HY-P77871
产品使用指南

AMIGO2 蛋白对于培养的小脑颗粒神经元的存活至关重要,介导同性和异性细胞与 AMIGO1 或 AMIGO3 的相互作用。其胞内结构域可能有助于信号转导,AMIGO2 表现出自结合能力以及与 AMIGO1 和 AMIGO3 蛋白的结合。AMIGO2 蛋白, Mouse (HEK293, His) 是重组的 AMIGO2 蛋白,由 HEK293 表达,带有 C-His 标签。AMIGO2 蛋白, Mouse (HEK293, His) 全长 359 个氨基酸,分子量为 55-65 kDa。

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描述

AMIGO2 Protein, crucial for the survival of cultured cerebellar granule neurons, mediates homophilic and heterophilic cell interactions with AMIGO1 or AMIGO3. Its intracellular domain may contribute to signal transduction, and AMIGO2 exhibits self-binding ability along with binding to AMIGO1 and AMIGO3 proteins. AMIGO2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived AMIGO2 protein, expressed by HEK293 , with C-His labeled tag. The total length of AMIGO2 Protein, Mouse (HEK293, His) is 359 a.a., with molecular weight of 55-65 kDa.

研究背景

AMIGO2 蛋白对于培养的小脑颗粒神经元的去极化依赖性存活至关重要。它在介导 AMIGO1 或 AMIGO3 的同性和异性细胞间相互作用中发挥着至关重要的作用。此外,据推测它通过其细胞内结构域有助于信号转导。此外,AMIGO2 能够结合自身以及 AMIGO1 和 AMIGO3 蛋白。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q80ZD9 (M39-T397)

基因 ID

105827  [NCBI]

蛋白结构
N-term
AMIGO2 (M39-T397)
Accession # Q80ZD9
His
C-term
同用名
Amphotein-induced protein 2; AMIGO-2; Alivin-1; DEGA; AMIGO2; ALI1; alivin 1
分子量

55-65 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

AMIGO2 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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AMIGO2 Protein, Mouse (HEK293, His)
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